rdf:type |
|
lifeskim:mentions |
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pubmed:issue |
12
|
pubmed:dateCreated |
2002-10-24
|
pubmed:abstractText |
The coupled reaction of N,N-dimethylaniline (DMA) with 4-aminoantipyrine (4-AAP) using superoxide anion radical (O2-) as oxidizing agent under the catalysis of horseradish peroxidase (HRP) was studied. Based on the reaction, O2- produced by irradiating Vitamin B2, (VB2) was spectrophotometricly determined at 554 nm. The linear range of this method was 1.8 x 10(-6)-1.2 x 10(-4) mol l(-1) with a detection limit of 5.3 x 10(-7) mol l(-1). The effect of interferences on the determination of O2- was investigated. The proposed method was successfully applied to the determination of superoxide dismutase (SOD) activity in human blood and mouse blood.
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pubmed:language |
eng
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pubmed:journal |
|
pubmed:citationSubset |
IM
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pubmed:chemical |
|
pubmed:status |
MEDLINE
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pubmed:month |
Oct
|
pubmed:issn |
1386-1425
|
pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:volume |
58
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
2557-62
|
pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:12396037-Adult,
pubmed-meshheading:12396037-Age Factors,
pubmed-meshheading:12396037-Ampyrone,
pubmed-meshheading:12396037-Aniline Compounds,
pubmed-meshheading:12396037-Animals,
pubmed-meshheading:12396037-Catalysis,
pubmed-meshheading:12396037-Horseradish Peroxidase,
pubmed-meshheading:12396037-Humans,
pubmed-meshheading:12396037-Mice,
pubmed-meshheading:12396037-Middle Aged,
pubmed-meshheading:12396037-Riboflavin,
pubmed-meshheading:12396037-Sensitivity and Specificity,
pubmed-meshheading:12396037-Spectrometry, Fluorescence,
pubmed-meshheading:12396037-Superoxide Dismutase,
pubmed-meshheading:12396037-Superoxides
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pubmed:year |
2002
|
pubmed:articleTitle |
Catalytic spectrofluorimetric determination of superoxide anion radical and superoxide dismutase activity using N,N-dimethylaniline as the substrate for horseradish peroxidase (HRP).
|
pubmed:affiliation |
Department of Chemistry, Shandong Normal University, Jinan, People's Republic of China. toplet@jn-public.sd.cninfo.net
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|