Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
2002-10-24
pubmed:abstractText
The coupled reaction of N,N-dimethylaniline (DMA) with 4-aminoantipyrine (4-AAP) using superoxide anion radical (O2-) as oxidizing agent under the catalysis of horseradish peroxidase (HRP) was studied. Based on the reaction, O2- produced by irradiating Vitamin B2, (VB2) was spectrophotometricly determined at 554 nm. The linear range of this method was 1.8 x 10(-6)-1.2 x 10(-4) mol l(-1) with a detection limit of 5.3 x 10(-7) mol l(-1). The effect of interferences on the determination of O2- was investigated. The proposed method was successfully applied to the determination of superoxide dismutase (SOD) activity in human blood and mouse blood.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1386-1425
pubmed:author
pubmed:issnType
Print
pubmed:volume
58
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2557-62
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2002
pubmed:articleTitle
Catalytic spectrofluorimetric determination of superoxide anion radical and superoxide dismutase activity using N,N-dimethylaniline as the substrate for horseradish peroxidase (HRP).
pubmed:affiliation
Department of Chemistry, Shandong Normal University, Jinan, People's Republic of China. toplet@jn-public.sd.cninfo.net
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't