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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
51
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pubmed:dateCreated |
2002-12-16
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pubmed:abstractText |
Ongoing sphingolipid synthesis is specifically required in vivo for the endoplasmic reticulum (ER) to Golgi transport of glycosylphosphatidylinositol (GPI)-anchored proteins. However, the sphingolipid intermediates that are required for transport nor their role(s) have been identified. Using stereoisomers of dihydrosphingosine, together with specific inhibitors and a mutant defective for sphingolipid synthesis, we now show that ceramides and/or inositol sphingolipids are indispensable for GPI-anchored protein transport. Furthermore, in the absence of sphingolipid synthesis, a significant fraction of GPI-anchored proteins is no longer associated tightly with the ER membrane. The loose membrane association is neither because of the lack of a GPI-anchor nor because of prolonged ER retention of GPI-anchored proteins. These results indicate that ceramides and/or inositol sphingolipids are required to stabilize the association of GPI-anchored proteins with membranes. They could act either by direct involvement as membrane components or as substrates for the remodeling of GPI lipid moieties.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
20
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pubmed:volume |
277
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
49538-44
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:12393888-Cell Cycle Proteins,
pubmed-meshheading:12393888-Cell Membrane,
pubmed-meshheading:12393888-Ceramides,
pubmed-meshheading:12393888-Endoplasmic Reticulum,
pubmed-meshheading:12393888-GPI-Linked Proteins,
pubmed-meshheading:12393888-Glycosylphosphatidylinositols,
pubmed-meshheading:12393888-Golgi Apparatus,
pubmed-meshheading:12393888-Lipid Metabolism,
pubmed-meshheading:12393888-Membrane Proteins,
pubmed-meshheading:12393888-Mutation,
pubmed-meshheading:12393888-Plasmids,
pubmed-meshheading:12393888-Protein Binding,
pubmed-meshheading:12393888-Protein Structure, Tertiary,
pubmed-meshheading:12393888-Protein Transport,
pubmed-meshheading:12393888-Sphingolipids,
pubmed-meshheading:12393888-Stereoisomerism,
pubmed-meshheading:12393888-Substrate Specificity,
pubmed-meshheading:12393888-Yeasts
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pubmed:year |
2002
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pubmed:articleTitle |
Sphingolipids are required for the stable membrane association of glycosylphosphatidylinositol-anchored proteins in yeast.
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pubmed:affiliation |
Biozentrum of the University of Basel, Klingelbergstrasse 70, Basel 4056, Switzerland.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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