|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
4
|
|
pubmed:dateCreated |
2003-1-31
|
|
pubmed:abstractText |
Dendritic cells (DCs) genetically engineered to overexpress CD95 (Fas) ligand (CD95L-DC) were proposed as tools to induce peripheral tolerance to alloantigens. Herein, we observed that CD95L-DC obtained after retroviral gene transfer in bone marrow (BM) precursors derived from CD95-deficient (lpr/lpr) mice elicit much stronger allospecific type 1 helper T-cell and cytotoxic T-cell activities than control DCs upon injection in vivo, although they induce lower T-cell responses in vitro. Indeed, a single injection of CD95L-DC prepared from C57BL/6 mice was sufficient to prime bm13 recipients for acute rejection of C57BL/6 skin allografts that were otherwise tolerated in the context of this single weak major histocompatibility complex (MHC) class I incompatibility. Massive neutrophil infiltrates depending on interleukin (IL)-1 signaling were observed at sites of CD95L-DC injection. Experiments in IL-1 receptor-deficient mice or in animals injected with depleting anti-Gr1 monoclonal antibody (mAb) established that neutrophil recruitment is required for the development of vigorous T-cell responses after injection of CD95L-DC in vivo.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
AIM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD95,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-2,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-4,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-5,
http://linkedlifedata.com/resource/pubmed/chemical/Isoantigens,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin-1
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Feb
|
|
pubmed:issn |
0006-4971
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
15
|
|
pubmed:volume |
101
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
1469-76
|
|
pubmed:dateRevised |
2008-11-21
|
|
pubmed:meshHeading |
pubmed-meshheading:12393481-Animals,
pubmed-meshheading:12393481-Antibodies, Monoclonal,
pubmed-meshheading:12393481-Antigens, CD95,
pubmed-meshheading:12393481-Dendritic Cells,
pubmed-meshheading:12393481-Graft Rejection,
pubmed-meshheading:12393481-Immune Tolerance,
pubmed-meshheading:12393481-Interferon-gamma,
pubmed-meshheading:12393481-Interleukin-2,
pubmed-meshheading:12393481-Interleukin-4,
pubmed-meshheading:12393481-Interleukin-5,
pubmed-meshheading:12393481-Isoantigens,
pubmed-meshheading:12393481-Mice,
pubmed-meshheading:12393481-Mice, Inbred BALB C,
pubmed-meshheading:12393481-Mice, Inbred C57BL,
pubmed-meshheading:12393481-Mice, Knockout,
pubmed-meshheading:12393481-Neutrophils,
pubmed-meshheading:12393481-Receptors, Interleukin-1,
pubmed-meshheading:12393481-Retroviridae,
pubmed-meshheading:12393481-Skin Transplantation,
pubmed-meshheading:12393481-T-Lymphocytes,
pubmed-meshheading:12393481-T-Lymphocytes, Cytotoxic,
pubmed-meshheading:12393481-T-Lymphocytes, Helper-Inducer,
pubmed-meshheading:12393481-Transfection
|
|
pubmed:year |
2003
|
|
pubmed:articleTitle |
Dendritic cells overexpressing CD95 (Fas) ligand elicit vigorous allospecific T-cell responses in vivo.
|
|
pubmed:affiliation |
Laboratory of Experimental Immunology, Université Libre de Bruxelles, Belgium.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
