Linked Life Data

12391181

Source:http://linkedlifedata.com/resource/pubmed/id/12391181

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
2002-10-22
pubmed:abstractText
Recently, it has been found that differentiation into osteoclasts is induced by TNF-alpha. In this study, we investigated the effect of IL-12 on TNF-alpha-mediated osteoclastogenesis. When mouse bone marrow cells were cultured with TNF-alpha, osteoclast-like cells were formed. When they were cultured with both TNF-alpha and IL-12, the number of adherent cells in the bone marrow cells decreased in an IL-12 dose-dependent manner. A combination of IL-12 and TNF-alpha was necessary to induce death of the adherent cells in this culture system. Apoptotic alterations, which were indicated by morphological changes such as cellular atrophy, nuclear and cellular fragmentation, and biochemical changes such as DNA fragmentation, were observed in the adherent cells. Apoptosis of the adherent cells was markedly inhibited by anti-Fas ligand (FasL) Ab. RT-PCR and FACS analyses revealed that TNF-alpha up-regulated Fas transcription to lead to Fas expression on the surfaces of the adherent cells, whereas IL-12 could not induce Fas on the cells. In contrast, IL-12 induced FasL transcription to lead to FasL expression on the surfaces of nonadherent bone marrow cells, whereas TNF-alpha could not induce FasL on the cells. These results implied that apoptosis of the adherent cells in bone marrow cells might be caused by interaction between TNF-alpha-induced Fas on the adherent cells and IL-12-induced FasL on the nonadherent cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
169
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4732-8
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:12391181-Animals, pubmed-meshheading:12391181-Antigens, CD95, pubmed-meshheading:12391181-Apoptosis, pubmed-meshheading:12391181-Benzimidazoles, pubmed-meshheading:12391181-Bone Marrow Cells, pubmed-meshheading:12391181-Cell Differentiation, pubmed-meshheading:12391181-Cell Nucleus, pubmed-meshheading:12391181-Cells, Cultured, pubmed-meshheading:12391181-DNA Fragmentation, pubmed-meshheading:12391181-Fas Ligand Protein, pubmed-meshheading:12391181-Fluorescent Dyes, pubmed-meshheading:12391181-Interleukin-12, pubmed-meshheading:12391181-Ligands, pubmed-meshheading:12391181-Macrophage Colony-Stimulating Factor, pubmed-meshheading:12391181-Macrophages, pubmed-meshheading:12391181-Male, pubmed-meshheading:12391181-Membrane Glycoproteins, pubmed-meshheading:12391181-Mice, pubmed-meshheading:12391181-Osteoclasts, pubmed-meshheading:12391181-Tumor Necrosis Factor-alpha
pubmed:year
2002
pubmed:articleTitle
Effect of IL-12 on TNF-alpha-mediated osteoclast formation in bone marrow cells: apoptosis mediated by Fas/Fas ligand interaction.
pubmed:affiliation
Division of Orthodontic and Biomedical Engineering, Department of Developmental and Reconstructive Medicine, Course of Medical and Dental Sciences, Nagasaki University Graduate School of Biomedical Sciences, Japan. hide@dh.nagasaki-u.ac.jp
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't