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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
22
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pubmed:dateCreated |
2002-10-22
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pubmed:abstractText |
Heat shock protein 72 (Hsp72) is thought to protect cells against cellular stress. The protective role of Hsp72 was investigated by determining the effect of this protein on the stress-activated protein kinase signaling pathways. Prior exposure of NIH 3T3 cells to mild heat shock (43 degrees C for 20 min) resulted in inhibition of H(2)O(2)-induced activation of apoptosis signal-regulating kinase 1 (ASK1). Overexpression of Hsp72 also inhibited H(2)O(2)-induced activation of ASK1 as well as that of downstream kinases in the p38 mitogen-activated protein kinase (MAPK) signaling cascade. Recombinant Hsp72 bound directly to ASK1 and inhibited ASK1 activity in vitro. Furthermore, coimmunoprecipitation analysis revealed a physical interaction between endogenous Hsp72 and ASK1 in NIH 3T3 cells exposed to mild heat shock. Hsp72 blocked both the homo-oligomerization of ASK1 and ASK1-dependent apoptosis. Hsp72 antisense oligonucleotides prevented the inhibitory effects of mild heat shock on H(2)O(2)-induced ASK1 activation and apoptosis. These observations suggest that Hsp72 functions as an endogenous inhibitor of ASK1.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/12391142-10064589,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12391142-10082520,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12391142-10199631,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12391142-10411906,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12391142-10557099,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12391142-10567231,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12391142-10594022,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12391142-10688666,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12391142-10734135,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12391142-10806214,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12391142-10849426,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12391142-10934466,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12391142-10934467,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12391142-10958679,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12391142-10982831,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12391142-11157751,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12391142-11278289,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12391142-11533664,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12391142-1394434,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12391142-1569948,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12391142-2188360,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12391142-2681224,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12391142-2858050,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12391142-3058716,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12391142-3282179,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12391142-7774586,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12391142-8409402,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12391142-8974401,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12391142-9218432,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12391142-9271409,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12391142-9497367,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12391142-9564042,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12391142-9651337,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12391142-9743501,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12391142-9774977,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12391142-9799222
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/HSP72 Heat-Shock Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Heat-Shock Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Hydrogen Peroxide,
http://linkedlifedata.com/resource/pubmed/chemical/MAP Kinase Kinase Kinase 5,
http://linkedlifedata.com/resource/pubmed/chemical/MAP Kinase Kinase Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/MAP3K5 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Map3k5 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Oligonucleotides, Antisense,
http://linkedlifedata.com/resource/pubmed/chemical/Oxidants,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0270-7306
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pubmed:author |
pubmed-author:ChoSsang-GooSG,
pubmed-author:ChoiEui-JuEJ,
pubmed-author:HuhSung-HoSH,
pubmed-author:HwangHyun SubHS,
pubmed-author:KangWoo JinWJ,
pubmed-author:KimChang KyunCK,
pubmed-author:KimEun KyungEK,
pubmed-author:KimMi-SungMS,
pubmed-author:KimMyung JinMJ,
pubmed-author:KimSunghoonS,
pubmed-author:KoYoung-GyuYG,
pubmed-author:LeeJae-SeonJS,
pubmed-author:NohKyung TaeKT,
pubmed-author:ParkHee-SaeHS,
pubmed-author:RyooKanghyunK,
pubmed-author:SeoJeong-SunJS
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pubmed:issnType |
Print
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pubmed:volume |
22
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
7721-30
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pubmed:dateRevised |
2011-11-2
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pubmed:meshHeading |
pubmed-meshheading:12391142-Humans,
pubmed-meshheading:12391142-Animals,
pubmed-meshheading:12391142-Mice,
pubmed-meshheading:12391142-Hot Temperature,
pubmed-meshheading:12391142-Hydrogen Peroxide,
pubmed-meshheading:12391142-Oxidants,
pubmed-meshheading:12391142-Protein Binding,
pubmed-meshheading:12391142-Enzyme Activation,
pubmed-meshheading:12391142-3T3 Cells,
pubmed-meshheading:12391142-Apoptosis,
pubmed-meshheading:12391142-Heat-Shock Proteins,
pubmed-meshheading:12391142-Recombinant Fusion Proteins,
pubmed-meshheading:12391142-MAP Kinase Kinase Kinases,
pubmed-meshheading:12391142-Oligonucleotides, Antisense,
pubmed-meshheading:12391142-Genes, Reporter,
pubmed-meshheading:12391142-HSP72 Heat-Shock Proteins,
pubmed-meshheading:12391142-MAP Kinase Kinase Kinase 5
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