Linked Life Data

1238980

Source:http://linkedlifedata.com/resource/pubmed/id/1238980

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1976-3-1
pubmed:abstractText
The present study was undertaken in anesthetized rats to evaluate the technique of efferent arteriolar and branch capillary microperfusion in the kidney. In contrary to previous suggestions, subsurface efferent arteriolar branches were demonstrated to occur prior to the surface site of insertion of the microperfusion pipette into an efferent arteriole. This finding, in addition to the presence of continued blood flow around the microperfusion pipette, provided an explanation for continued filtration and tubular reabsorption in nephrons under study. Moreover, additional microdissection studies demonstrated that a mean of 3.8 nephrons are surrounded by artificially perfused capillaries from a micropipette inserted into a single efferent arteriole. Thus, any obstructive effect of the microperfusion technique on post-glomerular blood flow might be expected to alter nephron function in only a minority of nephrons which are surrounded by artificial perfusate. The results also demonstrated the importance of avioding osmotic gradients between the animal's plasma and the artificial perfusate. A method is also described in which the correlation between in vivo diagrams and in vitro microdissections allows assessment of the completeness of the artificial perfusion of capillaries surrounding the surface loops of nephrons under study.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0031-6768
pubmed:author
pubmed:issnType
Print
pubmed:day
9
pubmed:volume
356
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
59-71
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1975
pubmed:articleTitle
Evaluation of peritubular capillary microperfusion method by morphological and functional studies.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.