Linked Life Data

12388332

Source:http://linkedlifedata.com/resource/pubmed/id/12388332

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2002-12-9
pubmed:abstractText
Organ volume is a critical parameter in morphometric analysis. The special problems of the lung as a nonsolid organ are overcome by tracheal instillation of fixatives at a constant airway pressure (P(aw)). Lung volume can change significantly after fixation as P(aw) change. To determine the variation of lung volume after fixation, we measured the volume of intact fixed lungs by serial immersion in saline (V(imm)) at selected time points, compared with measurements obtained by point counting [Cavalieri Principle (V(cav))] after tissue sectioning to release P(aw). V(imm) was systematically higher than V(cav) by 25% in dog lungs and 13% in guinea pig lungs (P = 0.0003 between species). This size-dependent variability reflects residual elastic recoil, refolding and/or crumpling of alveolar septa after fixation. V(imm) remained 14% higher than V(cav) in dog lungs even after pressure release. V(cav)/V(imm) was systematically lower in the upper than the lower strata of the same lung. We conclude that V(cav) measured on lung slices after relaxation of P(aw) more precisely represents the state of the tissue to be used for subsequent morphometric analysis, particularly for large lungs.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1040-0605
pubmed:author
pubmed:issnType
Print
pubmed:volume
284
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
L242-5
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
Variation of lung volume after fixation when measured by immersion or Cavalieri method.
pubmed:affiliation
Department of Internal Medicine, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390-9034, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S.