Source:http://linkedlifedata.com/resource/pubmed/id/12388130
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
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| pubmed:dateCreated |
2002-12-17
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| pubmed:abstractText |
We have generated transgenic mice that express green fluorescent protein (GFP) under the control of the mouse insulin I gene promoter (MIP). The MIP-GFP mice develop normally and are indistinguishable from control animals with respect to glucose tolerance and pancreatic insulin content. Histological studies showed that the MIP-GFP mice had normal islet architecture with coexpression of insulin and GFP in the beta-cells of all islets. We observed GFP expression in islets from embryonic day E13.5 through adulthood. Studies of beta-cell function revealed no difference in glucose-induced intracellular calcium mobilization between islets from transgenic and control animals. We prepared single-cell suspensions from both isolated islets and whole pancreas from MIP-GFP-transgenic mice and sorted the beta-cells by fluorescence-activated cell sorting based on their green fluorescence. These studies showed that 2.4 +/- 0.2% (n = 6) of the cells in the pancreas of newborn (P1) and 0.9 +/- 0.1% (n = 5) of 8-wk-old mice were beta-cells. The MIP-GFP-transgenic mouse may be a useful tool for studying beta-cell biology in normal and diabetic animals.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Glucose,
http://linkedlifedata.com/resource/pubmed/chemical/Green Fluorescent Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Insulin,
http://linkedlifedata.com/resource/pubmed/chemical/Luminescent Proteins
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| pubmed:status |
MEDLINE
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| pubmed:month |
Jan
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| pubmed:issn |
0193-1849
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
284
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
E177-83
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| pubmed:dateRevised |
2011-11-17
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| pubmed:meshHeading |
pubmed-meshheading:12388130-Aging,
pubmed-meshheading:12388130-Animals,
pubmed-meshheading:12388130-Blotting, Western,
pubmed-meshheading:12388130-Calcium,
pubmed-meshheading:12388130-Cell Count,
pubmed-meshheading:12388130-Female,
pubmed-meshheading:12388130-Flow Cytometry,
pubmed-meshheading:12388130-Gene Expression,
pubmed-meshheading:12388130-Glucose,
pubmed-meshheading:12388130-Glucose Tolerance Test,
pubmed-meshheading:12388130-Green Fluorescent Proteins,
pubmed-meshheading:12388130-Insulin,
pubmed-meshheading:12388130-Islets of Langerhans,
pubmed-meshheading:12388130-Luminescent Proteins,
pubmed-meshheading:12388130-Male,
pubmed-meshheading:12388130-Mice,
pubmed-meshheading:12388130-Mice, Transgenic,
pubmed-meshheading:12388130-Pancreas,
pubmed-meshheading:12388130-Promoter Regions, Genetic
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| pubmed:year |
2003
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| pubmed:articleTitle |
Transgenic mice with green fluorescent protein-labeled pancreatic beta -cells.
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| pubmed:affiliation |
Department of Medicine, The University of Chicago, Illinois 60637, USA. mhara@midway.uchicago.edu
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|