Linked Life Data

12387715

Source:http://linkedlifedata.com/resource/pubmed/id/12387715

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
2002-10-21
pubmed:abstractText
Glutamine synthetase (GS) has been purified from the cytosolic fraction of non-frozen human brain tissue. The purified GS migrated as a main band around 44 kD on reducing SDS-PAGE. Two-dimensional electrophoresis revealed heterogeneity within subunits of GS. The masses of eight different peptides from a tryptic digest of GS as measured by high resolution MALDI-MS matched with the respective masses from an in silico tryptic fingerprint of the Swiss-Prot database entry of human liver GS, proving that at least 24% of the primary sequences of GS from brain and liver are identical. Sedimentation equilibrium profiles obtained from analytical ultracentrifugation experiments at 10 degrees C showed that human brain GS is mainly octameric. The quaternary structure of human brain GS at 10 microM (subunit concentration) was not significantly affected by cations, such as magnesium (5 and 20 mM) or manganese (0.2 and 1 mM) within the range of pH 7.1-7.8.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0006-2979
pubmed:author
pubmed:issnType
Print
pubmed:volume
67
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1012-20
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2002
pubmed:articleTitle
Glutamine synthetase isolated from human brain: octameric structure and homology of partial primary structure with human liver glutamine synthetase.
pubmed:affiliation
Laboratory of Neurochemistry, Mental Health Research Center of Russian Academy of Medical Sciences, Moscow, 117152, Russia. boksha_irina@yahoo.co.uk
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't