Source:http://linkedlifedata.com/resource/pubmed/id/12387504
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Predicate | Object |
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rdf:type | |
lifeskim:mentions |
umls-concept:C0007634,
umls-concept:C0033268,
umls-concept:C0036098,
umls-concept:C0040203,
umls-concept:C0332293,
umls-concept:C0524816,
umls-concept:C0599209,
umls-concept:C1264633,
umls-concept:C1555707,
umls-concept:C1623048,
umls-concept:C1705851,
umls-concept:C1707391,
umls-concept:C2752151,
umls-concept:C2828366
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pubmed:issue |
2
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pubmed:dateCreated |
2002-10-21
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pubmed:abstractText |
A salivary gland extract (SGE) prepared from 5-days-fed Dermacentor reticulatus female ticks was fractionated by fast protein liquid chromatography (FPLC). The effect of three FPLC fractions selected on the basis of anti-interleukin 8 (anti-IL-8) activity on vesicular stomatitis virus (VSV) nucleocapsid (N) protein formation in mouse L-cells was determined. Infected 14C-labeled cells treated with the FPLC fractions were analyzed by two-dimensional (2D) electrophoresis. The yields of VSV N protein were evaluated by Imagemaster software analysis. Most noticeable was an increase in the N protein production after treatment with the fraction 39 corresponding to the major peak of the anti-IL-8 activity. The nature of the substance in SGE that was responsible for this effect remains unclear.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:issn |
0001-723X
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
46
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
117-20
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:12387504-Animals,
pubmed-meshheading:12387504-Cell Extracts,
pubmed-meshheading:12387504-Cell Fractionation,
pubmed-meshheading:12387504-Chromatography, Liquid,
pubmed-meshheading:12387504-Dermacentor,
pubmed-meshheading:12387504-Electrophoresis, Gel, Two-Dimensional,
pubmed-meshheading:12387504-Female,
pubmed-meshheading:12387504-Interleukin-8,
pubmed-meshheading:12387504-L Cells (Cell Line),
pubmed-meshheading:12387504-Mice,
pubmed-meshheading:12387504-Nucleocapsid,
pubmed-meshheading:12387504-Nucleocapsid Proteins,
pubmed-meshheading:12387504-Salivary Glands,
pubmed-meshheading:12387504-Vesicular stomatitis Indiana virus
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pubmed:year |
2002
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pubmed:articleTitle |
Vesicular stomatitis virus nucleocapsid protein production in cells treated with selected fast protein liquid chromatography fractions of tick salivary gland extracts.
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pubmed:affiliation |
Institute of Virology, Slovak Academy of Sciences, Bratislava, Slovak Republic.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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