| pubmed-article:12383645 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12383645 | lifeskim:mentions | umls-concept:C1135183 | lld:lifeskim |
| pubmed-article:12383645 | lifeskim:mentions | umls-concept:C0123759 | lld:lifeskim |
| pubmed-article:12383645 | lifeskim:mentions | umls-concept:C0024264 | lld:lifeskim |
| pubmed-article:12383645 | lifeskim:mentions | umls-concept:C0597357 | lld:lifeskim |
| pubmed-article:12383645 | lifeskim:mentions | umls-concept:C0678226 | lld:lifeskim |
| pubmed-article:12383645 | lifeskim:mentions | umls-concept:C0441889 | lld:lifeskim |
| pubmed-article:12383645 | lifeskim:mentions | umls-concept:C1280500 | lld:lifeskim |
| pubmed-article:12383645 | lifeskim:mentions | umls-concept:C1417683 | lld:lifeskim |
| pubmed-article:12383645 | lifeskim:mentions | umls-concept:C0439801 | lld:lifeskim |
| pubmed-article:12383645 | lifeskim:mentions | umls-concept:C0205251 | lld:lifeskim |
| pubmed-article:12383645 | pubmed:issue | 3-4 | lld:pubmed |
| pubmed-article:12383645 | pubmed:dateCreated | 2002-10-17 | lld:pubmed |
| pubmed-article:12383645 | pubmed:abstractText | The cytokine interleukin-12 (IL-12) is a key molecule in the regulation of CD4 + T cell development and specifically potentiates T helper 1 responses in mouse and man. However, biological effects mediated by IL-12 have not been well defined in pigs. Herein, recombinant porcine IL-12 (rPoIL-12) was expressed in a swine poxvirus system as a biologically active heterodimer and used to stimulate bovine or swine lymphoblast cells. After 3 days of incubation, only bovine blasts were responsive to the rPoIL-12 treatment as monitored by cell proliferation in several independent trials. Similarly, i.m. administration of rPoIL-12 in the hind leg of 3-week-old pigs indicated a reduction in the number of interferon-gamma (IFN-gamma) producing lymphocytes isolated from inguinal lymph nodes. The porcine IL-12R beta2 (IL-12Rbeta2) sequence was cloned and results generated by reverse transcriptase polymerase chain reaction (RT-PCR) demonstrated that the expression of IL-12R on porcine blasts as measured by the relative levels of IL-12Rbeta2 mRNA was less than that in bovine blasts and are in agreement with the reduced proliferation response of swine blast cells to rPoIL-12 treatment. Real time PCR analysis demonstrated that after PBMC stimulation, bovine blasts had an 11-fold increase in IL-12Rbeta2 mRNA levels while porcine blasts had almost no change. These data support a mechanism for IL-12 stimulation in swine inconsistent with that observed in conventional models. | lld:pubmed |
| pubmed-article:12383645 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12383645 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12383645 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12383645 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:12383645 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12383645 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12383645 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12383645 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12383645 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12383645 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12383645 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12383645 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12383645 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12383645 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:12383645 | pubmed:issn | 0165-2427 | lld:pubmed |
| pubmed-article:12383645 | pubmed:author | pubmed-author:CochranMM | lld:pubmed |
| pubmed-article:12383645 | pubmed:author | pubmed-author:LunneyJ KJK | lld:pubmed |
| pubmed-article:12383645 | pubmed:author | pubmed-author:ChiangCC | lld:pubmed |
| pubmed-article:12383645 | pubmed:author | pubmed-author:ValencicFF | lld:pubmed |
| pubmed-article:12383645 | pubmed:author | pubmed-author:WestonCC | lld:pubmed |
| pubmed-article:12383645 | pubmed:author | pubmed-author:UrbanJ FJF | lld:pubmed |
| pubmed-article:12383645 | pubmed:author | pubmed-author:JunkerDD | lld:pubmed |
| pubmed-article:12383645 | pubmed:author | pubmed-author:GasbarroVV | lld:pubmed |
| pubmed-article:12383645 | pubmed:author | pubmed-author:DawsonHH | lld:pubmed |
| pubmed-article:12383645 | pubmed:author | pubmed-author:ZarlengaDD | lld:pubmed |
| pubmed-article:12383645 | pubmed:author | pubmed-author:BeshahEE | lld:pubmed |
| pubmed-article:12383645 | pubmed:author | pubmed-author:Solano-Aguila... | lld:pubmed |
| pubmed-article:12383645 | pubmed:author | pubmed-author:VengroskiKK | lld:pubmed |
| pubmed-article:12383645 | pubmed:copyrightInfo | Copyright 2002 Elsevier Science B.V. | lld:pubmed |
| pubmed-article:12383645 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12383645 | pubmed:day | 28 | lld:pubmed |
| pubmed-article:12383645 | pubmed:volume | 89 | lld:pubmed |
| pubmed-article:12383645 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12383645 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12383645 | pubmed:pagination | 133-48 | lld:pubmed |
| pubmed-article:12383645 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
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| pubmed-article:12383645 | pubmed:year | 2002 | lld:pubmed |
| pubmed-article:12383645 | pubmed:articleTitle | Limited effect of recombinant porcine interleukin-12 on porcine lymphocytes due to a low level of IL-12 beta2 receptor. | lld:pubmed |
| pubmed-article:12383645 | pubmed:affiliation | Nutrient Requirement and Functions Laboratory, BHNRC-ARS-USDA, 10300 Baltimore Avenue, Building 307, Room 228, Beltsville, MD 20705, USA. solano-aguilar@ba.ars.usda.gov | lld:pubmed |
| pubmed-article:12383645 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:12383645 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| entrez-gene:397178 | entrezgene:pubmed | pubmed-article:12383645 | lld:entrezgene |
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