|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
3-4
|
|
pubmed:dateCreated |
2002-10-17
|
|
pubmed:abstractText |
The cytokine interleukin-12 (IL-12) is a key molecule in the regulation of CD4 + T cell development and specifically potentiates T helper 1 responses in mouse and man. However, biological effects mediated by IL-12 have not been well defined in pigs. Herein, recombinant porcine IL-12 (rPoIL-12) was expressed in a swine poxvirus system as a biologically active heterodimer and used to stimulate bovine or swine lymphoblast cells. After 3 days of incubation, only bovine blasts were responsive to the rPoIL-12 treatment as monitored by cell proliferation in several independent trials. Similarly, i.m. administration of rPoIL-12 in the hind leg of 3-week-old pigs indicated a reduction in the number of interferon-gamma (IFN-gamma) producing lymphocytes isolated from inguinal lymph nodes. The porcine IL-12R beta2 (IL-12Rbeta2) sequence was cloned and results generated by reverse transcriptase polymerase chain reaction (RT-PCR) demonstrated that the expression of IL-12R on porcine blasts as measured by the relative levels of IL-12Rbeta2 mRNA was less than that in bovine blasts and are in agreement with the reduced proliferation response of swine blast cells to rPoIL-12 treatment. Real time PCR analysis demonstrated that after PBMC stimulation, bovine blasts had an 11-fold increase in IL-12Rbeta2 mRNA levels while porcine blasts had almost no change. These data support a mechanism for IL-12 stimulation in swine inconsistent with that observed in conventional models.
|
|
pubmed:commentsCorrections |
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/IL12RB2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Il12rb2 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-12,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Subunits,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin-12
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Oct
|
|
pubmed:issn |
0165-2427
|
|
pubmed:author |
pubmed-author:BeshahEE,
pubmed-author:ChiangCC,
pubmed-author:CochranMM,
pubmed-author:DawsonHH,
pubmed-author:GasbarroVV,
pubmed-author:JunkerDD,
pubmed-author:LunneyJ KJK,
pubmed-author:Solano-AguilarG IGI,
pubmed-author:UrbanJ FJF,
pubmed-author:ValencicFF,
pubmed-author:VengroskiKK,
pubmed-author:WestonCC,
pubmed-author:ZarlengaDD
|
|
pubmed:copyrightInfo |
Copyright 2002 Elsevier Science B.V.
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
28
|
|
pubmed:volume |
89
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
133-48
|
|
pubmed:dateRevised |
2008-11-21
|
|
pubmed:meshHeading |
pubmed-meshheading:12383645-Amino Acid Sequence,
pubmed-meshheading:12383645-Animals,
pubmed-meshheading:12383645-Biological Assay,
pubmed-meshheading:12383645-Blotting, Western,
pubmed-meshheading:12383645-Cattle,
pubmed-meshheading:12383645-Cells, Cultured,
pubmed-meshheading:12383645-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:12383645-Gene Expression,
pubmed-meshheading:12383645-Humans,
pubmed-meshheading:12383645-Interferon-gamma,
pubmed-meshheading:12383645-Interleukin-12,
pubmed-meshheading:12383645-Lymphocyte Activation,
pubmed-meshheading:12383645-Molecular Sequence Data,
pubmed-meshheading:12383645-Protein Subunits,
pubmed-meshheading:12383645-RNA, Messenger,
pubmed-meshheading:12383645-Receptors, Interleukin,
pubmed-meshheading:12383645-Receptors, Interleukin-12,
pubmed-meshheading:12383645-Sequence Homology, Amino Acid,
pubmed-meshheading:12383645-Suipoxvirus,
pubmed-meshheading:12383645-Swine,
pubmed-meshheading:12383645-T-Lymphocytes
|
|
pubmed:year |
2002
|
|
pubmed:articleTitle |
Limited effect of recombinant porcine interleukin-12 on porcine lymphocytes due to a low level of IL-12 beta2 receptor.
|
|
pubmed:affiliation |
Nutrient Requirement and Functions Laboratory, BHNRC-ARS-USDA, 10300 Baltimore Avenue, Building 307, Room 228, Beltsville, MD 20705, USA. solano-aguilar@ba.ars.usda.gov
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
