Source:http://linkedlifedata.com/resource/pubmed/id/12378512
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
9
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| pubmed:dateCreated |
2002-10-14
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| pubmed:abstractText |
The INK4a-ARF locus, located on chromosome 9p21, encodes 2 cell cycle-regulatory proteins, p16(INKa) and p14(ARF), acting through the Rb-CDK4 and p53 pathways. This study was done to investigate the contribution of the INK4a-ARF locus in tumorigenesis of angiosarcoma of the liver. Alterations of p14(ARF), p16(INKa), and p53 in primary liver angiosarcoma from 19 patients were analyzed by methylation-specific polymerase chain reaction (MSP), restriction enzyme-related polymerase chain reaction (RE-PCR), microsatellite analysis, and DNA sequencing. As a control group, 12 angiosarcomas from other organs were analyzed. Promoter methylation of p14(ARF) was found in 5 of 19 cases (26%), and p16(INKa) showed aberrant promoter methylation in 12 of 19 cases (63%). One tumor (5%) had homozygous deletion of the INK4a-ARF locus. Methylation and deletion correlated with loss of mRNA transcription. Methylated p14(ARF) appeared in the context of a methylated p16(INKa) promoter in 3 cases of the 5 angiosarcomas methylated at p14(ARF). p14(ARF) aberrant methylation was not related to the presence of p53 mutations, which was detected in 6 of 19 (32%) cases. Alterations of the INK4a-ARF locus or p53 as were not established independent prognostic factors in these tumors. In conclusion, our data indicate that the INK4a-ARF locus is frequently inactivated in angiosarcoma of the liver and occurs independently of p53 mutations.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cyclin-Dependent Kinase Inhibitor...,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Neoplasm,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Suppressor Protein p14ARF,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Suppressor Protein p53,
http://linkedlifedata.com/resource/pubmed/chemical/Vinyl Chloride
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| pubmed:status |
MEDLINE
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| pubmed:month |
Sep
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| pubmed:issn |
0046-8177
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| pubmed:author | |
| pubmed:copyrightInfo |
Copyright 2002, Elsevier Science (USA). All rights reserved.
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| pubmed:issnType |
Print
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| pubmed:volume |
33
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
884-92
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| pubmed:dateRevised |
2005-11-17
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| pubmed:meshHeading |
pubmed-meshheading:12378512-Aged,
pubmed-meshheading:12378512-Aged, 80 and over,
pubmed-meshheading:12378512-Cyclin-Dependent Kinase Inhibitor p16,
pubmed-meshheading:12378512-DNA, Neoplasm,
pubmed-meshheading:12378512-DNA Methylation,
pubmed-meshheading:12378512-Female,
pubmed-meshheading:12378512-Hemangiosarcoma,
pubmed-meshheading:12378512-Humans,
pubmed-meshheading:12378512-Liver Neoplasms,
pubmed-meshheading:12378512-Loss of Heterozygosity,
pubmed-meshheading:12378512-Male,
pubmed-meshheading:12378512-Microsatellite Repeats,
pubmed-meshheading:12378512-Middle Aged,
pubmed-meshheading:12378512-Polymerase Chain Reaction,
pubmed-meshheading:12378512-Retrospective Studies,
pubmed-meshheading:12378512-Sequence Analysis, DNA,
pubmed-meshheading:12378512-Tissue Embedding,
pubmed-meshheading:12378512-Tumor Suppressor Protein p14ARF,
pubmed-meshheading:12378512-Tumor Suppressor Protein p53,
pubmed-meshheading:12378512-Vinyl Chloride
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| pubmed:year |
2002
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| pubmed:articleTitle |
Abnormalities of the ARF-p53 pathway in primary angiosarcomas of the liver.
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| pubmed:affiliation |
Institute of Occupational Medicine, University of Hannover, Germany.
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| pubmed:publicationType |
Journal Article
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