| pubmed-article:12376757 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12376757 | lifeskim:mentions | umls-concept:C0019704 | lld:lifeskim |
| pubmed-article:12376757 | lifeskim:mentions | umls-concept:C0522424 | lld:lifeskim |
| pubmed-article:12376757 | lifeskim:mentions | umls-concept:C0008109 | lld:lifeskim |
| pubmed-article:12376757 | lifeskim:mentions | umls-concept:C0020205 | lld:lifeskim |
| pubmed-article:12376757 | lifeskim:mentions | umls-concept:C0017280 | lld:lifeskim |
| pubmed-article:12376757 | lifeskim:mentions | umls-concept:C0871161 | lld:lifeskim |
| pubmed-article:12376757 | pubmed:issue | 10 | lld:pubmed |
| pubmed-article:12376757 | pubmed:dateCreated | 2002-10-11 | lld:pubmed |
| pubmed-article:12376757 | pubmed:abstractText | The nef genes of human and simian immunodeficiency viruses code for a membrane associated protein critical for AIDS development. SIVmac Nef presents C-terminal a 27 amino acid extension absent of HIV-1 Nef. To estimate the influence of this C-terminal domain on virus properties, we constructed viruses derived from SIVmac239 by replacing SIV nef with HIV-1 Lai nef gene (SHIV NefLai4) or with a sequence encoding a Nef fusion protein: HIV-1 Lai Nef/SIV Nef-Cterm (SHIV-Cterm). The recombinant viruses replicated efficiently in vitro in CEMx174 cells and in activated macaque PBMCs. The addition of SIV Nef C-terminal domain to HIV-1 Nef in SHIVNefLai4 did not change the in vitro properties of the chimeric virus, both viruses being more infectious than a nef deleted virus. Although the half-life of Nef fusion protein was augmented, SHIV-Cterm remained slightly less infectious than SIVmac239. | lld:pubmed |
| pubmed-article:12376757 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12376757 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12376757 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:12376757 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12376757 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12376757 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12376757 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12376757 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:12376757 | pubmed:issn | 0304-8608 | lld:pubmed |
| pubmed-article:12376757 | pubmed:author | pubmed-author:GloecklerLL | lld:pubmed |
| pubmed-article:12376757 | pubmed:author | pubmed-author:BeyerCC | lld:pubmed |
| pubmed-article:12376757 | pubmed:author | pubmed-author:LEEM CMC | lld:pubmed |
| pubmed-article:12376757 | pubmed:author | pubmed-author:AubertinA MAM | lld:pubmed |
| pubmed-article:12376757 | pubmed:author | pubmed-author:CecileAA | lld:pubmed |
| pubmed-article:12376757 | pubmed:author | pubmed-author:BertschCC | lld:pubmed |
| pubmed-article:12376757 | pubmed:author | pubmed-author:CluetDD | lld:pubmed |
| pubmed-article:12376757 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12376757 | pubmed:volume | 147 | lld:pubmed |
| pubmed-article:12376757 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12376757 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12376757 | pubmed:pagination | 1963-75 | lld:pubmed |
| pubmed-article:12376757 | pubmed:dateRevised | 2007-11-15 | lld:pubmed |
| pubmed-article:12376757 | pubmed:meshHeading | pubmed-meshheading:12376757... | lld:pubmed |
| pubmed-article:12376757 | pubmed:meshHeading | pubmed-meshheading:12376757... | lld:pubmed |
| pubmed-article:12376757 | pubmed:meshHeading | pubmed-meshheading:12376757... | lld:pubmed |
| pubmed-article:12376757 | pubmed:meshHeading | pubmed-meshheading:12376757... | lld:pubmed |
| pubmed-article:12376757 | pubmed:meshHeading | pubmed-meshheading:12376757... | lld:pubmed |
| pubmed-article:12376757 | pubmed:meshHeading | pubmed-meshheading:12376757... | lld:pubmed |
| pubmed-article:12376757 | pubmed:meshHeading | pubmed-meshheading:12376757... | lld:pubmed |
| pubmed-article:12376757 | pubmed:meshHeading | pubmed-meshheading:12376757... | lld:pubmed |
| pubmed-article:12376757 | pubmed:meshHeading | pubmed-meshheading:12376757... | lld:pubmed |
| pubmed-article:12376757 | pubmed:meshHeading | pubmed-meshheading:12376757... | lld:pubmed |
| pubmed-article:12376757 | pubmed:year | 2002 | lld:pubmed |
| pubmed-article:12376757 | pubmed:articleTitle | Properties of a chimeric simian-human immunodeficiency virus expressing an hybrid HIV-1 Nef/SIVmac Nef protein. | lld:pubmed |
| pubmed-article:12376757 | pubmed:affiliation | INSERM U544, Institut de Virologie, Université Louis Pasteur, Strasbourg, France. | lld:pubmed |
| pubmed-article:12376757 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:12376757 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:12376757 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:12376757 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:12376757 | lld:pubmed |
