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rdf:type |
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lifeskim:mentions |
umls-concept:C0035820,
umls-concept:C0039194,
umls-concept:C0085358,
umls-concept:C0205160,
umls-concept:C0456387,
umls-concept:C0567416,
umls-concept:C1332717,
umls-concept:C1413244,
umls-concept:C1706438,
umls-concept:C1749467,
umls-concept:C1948023,
umls-concept:C2698600
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pubmed:issue |
21
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pubmed:dateCreated |
2002-10-16
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pubmed:abstractText |
Crosslinking of the T cell receptor has been proposed to be a prerequisite for T cell activation. Although the evidence supports this notion for CD4 T cells, the situation for CD8 T cells is less clear. Soluble class I monomers have been used to determine activation requirements in vitro with contradictory results. The possibility of transfer of peptide from soluble class I molecules onto class I molecules present on the surface of CD8 T cells, with ensuing presentation to other CD8 T cells, has been widely ignored. We show that monomeric and tetrameric class I molecules as well as free peptide can stimulate naive CD8 T cells in vitro. We generate and characterize CD8 T cells that express the OT-I T cell receptor (for K(b)/SIINFEKL) yet lack K(b) and D(b) molecules, and show that their activation requirements differ from their class I positive counterparts when stimulated with soluble K(b) molecules. By eliminating the confounding effect of peptide transfer, we unmask the true activation requirements for naive CD8 T cells and show that multivalent engagement of T cell receptors, as well as costimulation, is required for optimal stimulation.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/12374858-10072075,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12374858-10358766,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12374858-10542149,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12374858-10637277,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12374858-10640733,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12374858-10755611,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12374858-10892729,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12374858-11404484,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12374858-11420039,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12374858-11441088,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12374858-11465114,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12374858-11485738,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12374858-11606747,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12374858-2123347,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12374858-7600293,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12374858-7629504,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12374858-8287475,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12374858-8335901,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12374858-8673703,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12374858-8691128,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12374858-8810254,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12374858-8955188,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12374858-9531279,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12374858-9770513,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12374858-9806632,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12374858-9806633,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12374858-9806634
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Egg Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/H-2 Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/H-2Kb protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Histocompatibility Antigens Class I,
http://linkedlifedata.com/resource/pubmed/chemical/OVA-8,
http://linkedlifedata.com/resource/pubmed/chemical/Ovalbumin,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/Peptides,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, T-Cell
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
99
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
13735-40
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:12374858-Adoptive Transfer,
pubmed-meshheading:12374858-Amino Acid Sequence,
pubmed-meshheading:12374858-Animals,
pubmed-meshheading:12374858-Antigen Presentation,
pubmed-meshheading:12374858-CD8-Positive T-Lymphocytes,
pubmed-meshheading:12374858-Egg Proteins,
pubmed-meshheading:12374858-H-2 Antigens,
pubmed-meshheading:12374858-Histocompatibility Antigens Class I,
pubmed-meshheading:12374858-Lymphocyte Activation,
pubmed-meshheading:12374858-Mice,
pubmed-meshheading:12374858-Mice, Transgenic,
pubmed-meshheading:12374858-Ovalbumin,
pubmed-meshheading:12374858-Peptide Fragments,
pubmed-meshheading:12374858-Peptides,
pubmed-meshheading:12374858-Phenotype,
pubmed-meshheading:12374858-Protein Structure, Quaternary,
pubmed-meshheading:12374858-Receptors, Antigen, T-Cell,
pubmed-meshheading:12374858-Solubility
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pubmed:year |
2002
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pubmed:articleTitle |
Class I negative CD8 T cells reveal the confounding role of peptide-transfer onto CD8 T cells stimulated with soluble H2-Kb molecules.
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pubmed:affiliation |
Department of Pathology, Harvard Medical School, Boston, MA 02115, USA.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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