Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
21
pubmed:dateCreated
2002-10-10
pubmed:abstractText
IS150, a member of the widespread IS3 family, contains two consecutive out-of-phase open reading frames, orfA and orfB, that partially overlap. These open reading frames encode three proteins, InsA, InsB, and the InsAB protein, which is jointly encoded by both open reading frames by means of programmed translational frameshifting. We demonstrate that the InsAB protein represents the IS150 element's transposase. In vivo, the wild-type IS150 element generates circular excision products and linear IS150 molecules. Circular and linear species have previously been detected with mutant derivatives of other members of the IS3 family. Our finding supports the assumption that these products represent true transposition intermediates of members of this family. Analysis of the molecular nature of these two species suggested that the circular forms are precursors of the linear molecules. Elimination of InsA synthesis within the otherwise intact element led to accumulation of large amounts of the linear species, indicating that the primary role of InsA may be to prevent abortive production of the linear species and to couple generation of these species to productive insertion events.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/12374815-10320583, http://linkedlifedata.com/resource/pubmed/commentcorrection/12374815-10556026, http://linkedlifedata.com/resource/pubmed/commentcorrection/12374815-10760133, http://linkedlifedata.com/resource/pubmed/commentcorrection/12374815-11598022, http://linkedlifedata.com/resource/pubmed/commentcorrection/12374815-1334464, http://linkedlifedata.com/resource/pubmed/commentcorrection/12374815-1653413, http://linkedlifedata.com/resource/pubmed/commentcorrection/12374815-1660923, http://linkedlifedata.com/resource/pubmed/commentcorrection/12374815-16886, http://linkedlifedata.com/resource/pubmed/commentcorrection/12374815-2163395, http://linkedlifedata.com/resource/pubmed/commentcorrection/12374815-2548993, http://linkedlifedata.com/resource/pubmed/commentcorrection/12374815-2553270, http://linkedlifedata.com/resource/pubmed/commentcorrection/12374815-2841644, http://linkedlifedata.com/resource/pubmed/commentcorrection/12374815-2985470, http://linkedlifedata.com/resource/pubmed/commentcorrection/12374815-4568763, http://linkedlifedata.com/resource/pubmed/commentcorrection/12374815-4894690, http://linkedlifedata.com/resource/pubmed/commentcorrection/12374815-7752887, http://linkedlifedata.com/resource/pubmed/commentcorrection/12374815-7932694, http://linkedlifedata.com/resource/pubmed/commentcorrection/12374815-8107082, http://linkedlifedata.com/resource/pubmed/commentcorrection/12374815-8107136, http://linkedlifedata.com/resource/pubmed/commentcorrection/12374815-8550559, http://linkedlifedata.com/resource/pubmed/commentcorrection/12374815-8824609, http://linkedlifedata.com/resource/pubmed/commentcorrection/12374815-8950268, http://linkedlifedata.com/resource/pubmed/commentcorrection/12374815-9214651, http://linkedlifedata.com/resource/pubmed/commentcorrection/12374815-9302014, http://linkedlifedata.com/resource/pubmed/commentcorrection/12374815-9413996, http://linkedlifedata.com/resource/pubmed/commentcorrection/12374815-9463394, http://linkedlifedata.com/resource/pubmed/commentcorrection/12374815-9477115, http://linkedlifedata.com/resource/pubmed/commentcorrection/12374815-9729608, http://linkedlifedata.com/resource/pubmed/commentcorrection/12374815-9761671
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0021-9193
pubmed:author
pubmed:issnType
Print
pubmed:volume
184
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
5833-41
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2002
pubmed:articleTitle
Escherichia coli insertion sequence IS150: transposition via circular and linear intermediates.
pubmed:affiliation
Institut für Biologie III, Universität, Schänzlestrasse 1, D-79104 Freiburg, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't