|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
51
|
|
pubmed:dateCreated |
2002-12-16
|
|
pubmed:abstractText |
Production of cytokines is one of the major mechanisms employed by CD4(+) T cells to coordinate immune responses. Although the molecular mechanisms controlling T cell cytokine production have been extensively studied, the factors that endow T cells with their ability to produce unique sets of cytokines have not been fully characterized. Interferon regulatory factor (IRF)-4 is a lymphoid-restricted member of the interferon regulatory factor family of transcriptional regulators, whose deficiency leads to a profound impairment in the ability of mature CD4(+) T cells to produce cytokines. In these studies, we have investigated the mechanisms employed by IRF-4 to control cytokine synthesis. We demonstrate that stable expression of IRF-4 in Jurkat T cells not only leads to a strong enhancement in the synthesis of interleukin (IL)-2, but also enables these cells to start producing considerable amounts of IL-4, IL-10, and IL-13. Transient transfection assays indicate that IRF-4 can transactivate luciferase reporter constructs driven by either the human IL-2 or the human IL-4 promoter. A detailed analysis of the effects of IRF-4 on the IL-4 promoter reveals that IRF-4 binds to a site adjacent to a functionally important NFAT binding element and that IRF-4 cooperates with NFATc1. These studies thus support the notion that IRF-4 represents one of the lymphoid-specific components that control the ability of T lymphocytes to produce a distinctive array of cytokines.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon Regulatory Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-10,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-13,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-2,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-4,
http://linkedlifedata.com/resource/pubmed/chemical/Luciferases,
http://linkedlifedata.com/resource/pubmed/chemical/NFATC Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/NFATC1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Nfatc1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Oligonucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/interferon regulatory factor-4
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Dec
|
|
pubmed:issn |
0021-9258
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
20
|
|
pubmed:volume |
277
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
49238-46
|
|
pubmed:dateRevised |
2008-11-21
|
|
pubmed:meshHeading |
pubmed-meshheading:12374808-Animals,
pubmed-meshheading:12374808-Blotting, Western,
pubmed-meshheading:12374808-CD4-Positive T-Lymphocytes,
pubmed-meshheading:12374808-Cell Line,
pubmed-meshheading:12374808-Cell Lineage,
pubmed-meshheading:12374808-Cloning, Molecular,
pubmed-meshheading:12374808-Cytokines,
pubmed-meshheading:12374808-DNA,
pubmed-meshheading:12374808-DNA, Complementary,
pubmed-meshheading:12374808-DNA-Binding Proteins,
pubmed-meshheading:12374808-Humans,
pubmed-meshheading:12374808-Interferon Regulatory Factors,
pubmed-meshheading:12374808-Interleukin-10,
pubmed-meshheading:12374808-Interleukin-13,
pubmed-meshheading:12374808-Interleukin-2,
pubmed-meshheading:12374808-Interleukin-4,
pubmed-meshheading:12374808-Jurkat Cells,
pubmed-meshheading:12374808-Luciferases,
pubmed-meshheading:12374808-Mice,
pubmed-meshheading:12374808-Mice, Inbred C57BL,
pubmed-meshheading:12374808-Mice, Knockout,
pubmed-meshheading:12374808-NFATC Transcription Factors,
pubmed-meshheading:12374808-Nuclear Proteins,
pubmed-meshheading:12374808-Oligonucleotides,
pubmed-meshheading:12374808-Promoter Regions, Genetic,
pubmed-meshheading:12374808-Protein Binding,
pubmed-meshheading:12374808-RNA, Messenger,
pubmed-meshheading:12374808-T-Lymphocytes,
pubmed-meshheading:12374808-Transcription Factors,
pubmed-meshheading:12374808-Transcriptional Activation,
pubmed-meshheading:12374808-Transfection
|
|
pubmed:year |
2002
|
|
pubmed:articleTitle |
Modulation of T cell cytokine production by interferon regulatory factor-4.
|
|
pubmed:affiliation |
Department of Medicine, Columbia University, 630 West 168th Street, New York, NY 10032, USA.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
