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rdf:type |
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lifeskim:mentions |
umls-concept:C0016030,
umls-concept:C0017262,
umls-concept:C0017337,
umls-concept:C0034693,
umls-concept:C0034721,
umls-concept:C0035820,
umls-concept:C0185117,
umls-concept:C0205160,
umls-concept:C0220905,
umls-concept:C0250414,
umls-concept:C1414314,
umls-concept:C1514559,
umls-concept:C2911684
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pubmed:issue |
12
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pubmed:dateCreated |
2002-10-10
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pubmed:abstractText |
The early growth response 1 (Egr-1) gene product is a transcription factor that functions as an oikis factor. Loss of Egr-1 expression is closely associated with tumor formation. Phospholipase Cgamma1 (PLCgamma1) is overexpressed in some tumors, and its overexpression causes anchorage-independent growth. Here we report that overexpression of PLCgamma1 and SH2-SH3 domain of PLCgamma1 decreased induction of Egr-1 and the Egr-1-regulated genes TSP-1 and PAI-1. Results from the nuclear run-on assay and transfection experiment with the proximal 455 base pair region of the Egr-1 promoter (-454 to +1) showed that Egr-1 transcriptional activity was suppressed in PLCgamma1-3Y1 cells whereas decay of Egr-1 mRNA was similar in both cell lines. Serum response element- and ternary complex factor Elk-1-mediated transcriptional activation of the reporter gene in response to EGF were also inhibited in PLCgamma1-3Y1 cells. Pretreatment with the protein synthesis inhibitor cycloheximide (CHX) partially abrogated the serum-induced suppression of Egr-1 transcription in PLCgamma1-3Y1 cells, suggesting that a CHX-sensitive factor(s) is involved in the suppression of Egr-1 transcription in PLCgamma1-3Y1 cells. Our results demonstrated that overexpression of PLCgamma1 functions as a negative modulator of the tumor suppressor Egr-1 gene expression, possibly through inhibition of Elk-1-dependent transcriptional activity.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Culture Media, Serum-Free,
http://linkedlifedata.com/resource/pubmed/chemical/Cycloheximide,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Dactinomycin,
http://linkedlifedata.com/resource/pubmed/chemical/EGR1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/ELK1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Early Growth Response Protein 1,
http://linkedlifedata.com/resource/pubmed/chemical/Egr1 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Elk1 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Epidermal Growth Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Immediate-Early Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Isoenzymes,
http://linkedlifedata.com/resource/pubmed/chemical/Phospholipase C gamma,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Type C Phospholipases,
http://linkedlifedata.com/resource/pubmed/chemical/ets-Domain Protein Elk-1
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
1530-6860
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pubmed:author |
pubmed-author:BaeSun SikSS,
pubmed-author:ChangJong-SooJS,
pubmed-author:HanMi YoungMY,
pubmed-author:HyunDae SungDS,
pubmed-author:KIMO SOS,
pubmed-author:KimJung-HyeJH,
pubmed-author:KimMyung JongMJ,
pubmed-author:KimYong SikYS,
pubmed-author:KimYoung-HoYH,
pubmed-author:KoJesangJ,
pubmed-author:LeeYoung HanYH,
pubmed-author:MinDo SikDS,
pubmed-author:NaDoe SunDS,
pubmed-author:ShinSoon YoungSY,
pubmed-author:SuhPann-GhillPG
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pubmed:issnType |
Electronic
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pubmed:volume |
16
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1504-14
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:12374773-Animals,
pubmed-meshheading:12374773-Cell Line,
pubmed-meshheading:12374773-Culture Media, Serum-Free,
pubmed-meshheading:12374773-Cycloheximide,
pubmed-meshheading:12374773-DNA-Binding Proteins,
pubmed-meshheading:12374773-Dactinomycin,
pubmed-meshheading:12374773-Early Growth Response Protein 1,
pubmed-meshheading:12374773-Epidermal Growth Factor,
pubmed-meshheading:12374773-Fibroblasts,
pubmed-meshheading:12374773-Gene Expression Regulation,
pubmed-meshheading:12374773-Humans,
pubmed-meshheading:12374773-Immediate-Early Proteins,
pubmed-meshheading:12374773-Isoenzymes,
pubmed-meshheading:12374773-Phospholipase C gamma,
pubmed-meshheading:12374773-Plasmids,
pubmed-meshheading:12374773-Promoter Regions, Genetic,
pubmed-meshheading:12374773-Proto-Oncogene Proteins,
pubmed-meshheading:12374773-RNA, Messenger,
pubmed-meshheading:12374773-Rats,
pubmed-meshheading:12374773-Transcription Factors,
pubmed-meshheading:12374773-Transcriptional Activation,
pubmed-meshheading:12374773-Tumor Cells, Cultured,
pubmed-meshheading:12374773-Type C Phospholipases,
pubmed-meshheading:12374773-ets-Domain Protein Elk-1,
pubmed-meshheading:12374773-src Homology Domains
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pubmed:year |
2002
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pubmed:articleTitle |
Negative regulatory role of overexpression of PLC gamma 1 in the expression of early growth response 1 gene in rat 3Y1 fibroblasts.
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pubmed:affiliation |
Department of Biochemistry and Molecular Biology, College of Medicine, Yeungnam University, Daegu, Korea 705-717.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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