Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
20
pubmed:dateCreated
2002-10-10
pubmed:abstractText
Three gene products that form independent mechanosensitive channel activities have been identified in Escherichia coli. Two of these, MscL and MscS, play a vital role in allowing the cell to survive acute hypotonic stress. Much less is known of the third protein, MscK (KefA). Here, we characterize the MscK channel activity and compare it with the activity of its structural and functional homologue, MscS. While both show a slight anionic preference, MscK appears to be more sensitive to membrane tension. In addition, MscK, but not MscS activity appears to be regulated by external ionic environment, requiring not only membrane tension but also high concentrations of external K(+), NH(4)(+), Rb(+) or Cs(+) to gate; no activity is observed with Na(+), Li(+) or N-methyl-D-glucamine (NMDG). An MscK gain-of-function mutant gates spontaneously in the presence of K(+) or similar ions, and will gate in the presence of Na(+), Li(+) and NMDG, but only when stimulated by membrane tension. Increased sensitivity and the highly regulated nature of MscK suggest a more specialized physiological role than other bacterial mechanosensitive channels.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/12374733-10051655, http://linkedlifedata.com/resource/pubmed/commentcorrection/12374733-10102934, http://linkedlifedata.com/resource/pubmed/commentcorrection/12374733-10202137, http://linkedlifedata.com/resource/pubmed/commentcorrection/12374733-10498951, http://linkedlifedata.com/resource/pubmed/commentcorrection/12374733-10512816, http://linkedlifedata.com/resource/pubmed/commentcorrection/12374733-10846181, http://linkedlifedata.com/resource/pubmed/commentcorrection/12374733-11016946, http://linkedlifedata.com/resource/pubmed/commentcorrection/12374733-11217861, http://linkedlifedata.com/resource/pubmed/commentcorrection/12374733-11274342, http://linkedlifedata.com/resource/pubmed/commentcorrection/12374733-11325722, http://linkedlifedata.com/resource/pubmed/commentcorrection/12374733-11463635, http://linkedlifedata.com/resource/pubmed/commentcorrection/12374733-11574473, http://linkedlifedata.com/resource/pubmed/commentcorrection/12374733-11679069, http://linkedlifedata.com/resource/pubmed/commentcorrection/12374733-11742979, http://linkedlifedata.com/resource/pubmed/commentcorrection/12374733-11875573, http://linkedlifedata.com/resource/pubmed/commentcorrection/12374733-11972047, http://linkedlifedata.com/resource/pubmed/commentcorrection/12374733-11985727, http://linkedlifedata.com/resource/pubmed/commentcorrection/12374733-12010487, http://linkedlifedata.com/resource/pubmed/commentcorrection/12374733-12015316, http://linkedlifedata.com/resource/pubmed/commentcorrection/12374733-12080120, http://linkedlifedata.com/resource/pubmed/commentcorrection/12374733-1710939, http://linkedlifedata.com/resource/pubmed/commentcorrection/12374733-1783908, http://linkedlifedata.com/resource/pubmed/commentcorrection/12374733-2436228, http://linkedlifedata.com/resource/pubmed/commentcorrection/12374733-2460920, http://linkedlifedata.com/resource/pubmed/commentcorrection/12374733-329878, http://linkedlifedata.com/resource/pubmed/commentcorrection/12374733-7511799, http://linkedlifedata.com/resource/pubmed/commentcorrection/12374733-7595939, http://linkedlifedata.com/resource/pubmed/commentcorrection/12374733-7715454, http://linkedlifedata.com/resource/pubmed/commentcorrection/12374733-8661505, http://linkedlifedata.com/resource/pubmed/commentcorrection/12374733-8661775, http://linkedlifedata.com/resource/pubmed/commentcorrection/12374733-8804105, http://linkedlifedata.com/resource/pubmed/commentcorrection/12374733-8876191, http://linkedlifedata.com/resource/pubmed/commentcorrection/12374733-8890153, http://linkedlifedata.com/resource/pubmed/commentcorrection/12374733-9004483, http://linkedlifedata.com/resource/pubmed/commentcorrection/12374733-9074781, http://linkedlifedata.com/resource/pubmed/commentcorrection/12374733-9074782, http://linkedlifedata.com/resource/pubmed/commentcorrection/12374733-9632260, http://linkedlifedata.com/resource/pubmed/commentcorrection/12374733-9736761, http://linkedlifedata.com/resource/pubmed/commentcorrection/12374733-9856938, http://linkedlifedata.com/resource/pubmed/commentcorrection/12374733-9916243
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0261-4189
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
21
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
5323-30
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2002
pubmed:articleTitle
Ionic regulation of MscK, a mechanosensitive channel from Escherichia coli.
pubmed:affiliation
Department of Physiology, University of Texas Southwestern Medical Center, Dallas, TX 75390-9040, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't