Linked Life Data

12373069

Source:http://linkedlifedata.com/resource/pubmed/id/12373069

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2002-10-9
pubmed:abstractText
The use of mammalian lung surfactant extracts has sharply reduced mortality and morbidity from respiratory distress syndrome in premature infants. Synthesis of surfactant protein B and C (SP-B and SP-C) analogues may lead the way to a synthetic surfactant preparation. Dimeric SP-B(1-25) (dSP-B(1-25)) is based on the N-terminal domain of human SP-B and SP-Cfc is a modified human SP-C in which a single phenylalanine is substituted for a palmitoylated cysteine residue in the N-terminal segment (Phe-4 > Cys-4 variant). We tested the effects of synthetic surfactants with 1 or 2% dSP-B(1-25) and 1% SP-Cfc on lung function in surfactant-deficient rats. Four experimental surfactant preparations were prepared by mixing 1% dSP-B(1-25), 2% dSP-B(1-25), 1% dSP-B(1-25) +1% SP-Cfc, and 2% dSP-B(1-25) +1% SP-Cfc with phospholipids (PL). PL and Survanta, a bovine lung extract, were controls. Groups of 8 rats were ventilated, lavaged until surfactant deficiency, and treated with 100 mg/kg surfactant. Arterial blood gas values and dynamic compliance were measured every 15 min and after 2 h of ventilation, the rats were killed and pressure-volume curves performed. Oxygenation improved quickly after instillation of surfactant with synthetic peptides and Survanta. Oxygenation and lung volumes were consistently higher in the 2% than in the 1% dSP-B(1-25) groups. Addition of 1% SP-Cfc to the synthetic surfactants further improved oxygenation and lung volume, but to a lesser extent than increasing the dSP-B(1-25) content from 1 to 2%. These data indicate that improvements in oxygenation and lung volume in lavaged rats are dependent on the concentration of dSP-B(1-25) in the surfactant preparation and that the presence of SP-Cfc has a relative minor effect on these parameters.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0006-3126
pubmed:author
pubmed:copyrightInfo
Copyright 2002 S. Karger AG, Basel
pubmed:issnType
Print
pubmed:volume
82
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
181-7
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:12373069-Amino Acid Sequence, pubmed-meshheading:12373069-Animals, pubmed-meshheading:12373069-Biological Agents, pubmed-meshheading:12373069-Dimerization, pubmed-meshheading:12373069-Disease Models, Animal, pubmed-meshheading:12373069-Humans, pubmed-meshheading:12373069-Lung, pubmed-meshheading:12373069-Lung Diseases, pubmed-meshheading:12373069-Lung Volume Measurements, pubmed-meshheading:12373069-Male, pubmed-meshheading:12373069-Molecular Sequence Data, pubmed-meshheading:12373069-Peptide Fragments, pubmed-meshheading:12373069-Pulmonary Surfactant-Associated Protein B, pubmed-meshheading:12373069-Pulmonary Surfactant-Associated Protein C, pubmed-meshheading:12373069-Pulmonary Surfactant-Associated Proteins, pubmed-meshheading:12373069-Pulmonary Surfactants, pubmed-meshheading:12373069-Rats, pubmed-meshheading:12373069-Rats, Sprague-Dawley, pubmed-meshheading:12373069-Respiration, Artificial, pubmed-meshheading:12373069-Therapeutic Irrigation
pubmed:year
2002
pubmed:articleTitle
Surfactant with SP-B and SP-C analogues improves lung function in surfactant-deficient rats.
pubmed:affiliation
Harbor-University of California Los Angeles Research & Education Institute, Torrance, Calif 90502, USA. fjwalther@aol.com
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S.