Linked Life Data

12372788

Source:http://linkedlifedata.com/resource/pubmed/id/12372788

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2002-10-9
pubmed:abstractText
The apical- and basolateral-specific distribution of target soluble N-ethylmaleimide-sensitive factor attachment protein receptors (t-SNAREs) of the syntaxin family appear to be critical for polarity in epithelial cells. To test whether differential SNARE expression and/or subcellular localization may contribute to the known diversity of trafficking phenotypes of epithelial cell types in vivo, we have investigated the distribution of syntaxins 2, 3, and 4 in epithelial cells along the renal tubule. Syntaxins 3 and 4 are restricted to the apical and basolateral domains, respectively, in all cell types, indicating that their mutually exclusive localizations are important for cell polarity. The expression level of syntaxin 3 is highly variable, depending on the cell type, suggesting that it is regulated in concert with the cellular requirement for apical exocytic pathways. While syntaxin 4 localizes all along the basal and lateral plasma membrane domains in vivo, it is restricted to the lateral membrane in Madin-Darby canine kidney (MDCK) cells in two-dimensional monolayer culture. When cultured as cysts in collagen, however, MDCK cells target syntaxin 4 correctly to the basal and lateral membranes. Unexpectedly, the polarity of syntaxin 2 is inverted between different tubule cell types, suggesting a role in establishing plasticity of targeting. The vesicle-associated (v)-SNARE endobrevin is highly expressed in intercalated cells and colocalizes with the H(+)-ATPase in alpha- but not beta-intercalated cells, suggesting its involvement in H(+)-ATPase trafficking in the former cell type. These results suggest that epithelial membrane trafficking phenotypes in vivo are highly variable and that different cell types express or localize SNARE proteins differentially as a mechanism to achieve this variability.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1931-857X
pubmed:author
pubmed:issnType
Print
pubmed:volume
283
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
F1111-22
pubmed:dateRevised
2011-4-28
pubmed:meshHeading
pubmed-meshheading:12372788-Animals, pubmed-meshheading:12372788-Antibody Specificity, pubmed-meshheading:12372788-Antigens, Surface, pubmed-meshheading:12372788-Cell Line, pubmed-meshheading:12372788-Cell Polarity, pubmed-meshheading:12372788-Epithelial Cells, pubmed-meshheading:12372788-Gene Expression, pubmed-meshheading:12372788-Kidney, pubmed-meshheading:12372788-Male, pubmed-meshheading:12372788-Membrane Fusion, pubmed-meshheading:12372788-Membrane Proteins, pubmed-meshheading:12372788-Nerve Tissue Proteins, pubmed-meshheading:12372788-Phenotype, pubmed-meshheading:12372788-Protein Transport, pubmed-meshheading:12372788-Qa-SNARE Proteins, pubmed-meshheading:12372788-R-SNARE Proteins, pubmed-meshheading:12372788-Rabbits, pubmed-meshheading:12372788-Rats, pubmed-meshheading:12372788-Rats, Sprague-Dawley, pubmed-meshheading:12372788-SNARE Proteins, pubmed-meshheading:12372788-Syntaxin 1, pubmed-meshheading:12372788-Vesicular Transport Proteins
pubmed:year
2002
pubmed:articleTitle
SNARE expression and localization in renal epithelial cells suggest mechanism for variability of trafficking phenotypes.
pubmed:affiliation
Department of Cell Biology, Lerner Research Institute, and Urological Institute, The Cleveland Clinic, Cleveland, Ohio 44195, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't