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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
2002-10-9
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pubmed:abstractText |
TAK1 mitogen-activated protein kinase kinase kinase (MAP3K) is activated by its specific activator, TAK1-binding protein 1 (TAB1). A constitutively active TAK1 mutant has not yet been generated due to the indispensable requirement of TAB1 for TAK1 kinase activity. In this study, we generated a novel constitutively active TAK1 by fusing its kinase domain to the minimal TAK1-activation domain of TAB1. Co-immunoprecipitation assay demonstrated that these domains interacted intra-molecularly. The TAK1-TAB1 fusion protein showed a significant MAP3K activity in vitro and activated c-Jun N-terminal kinase/p38 MAPKs and IkappaB kinase in vivo, which was followed by increased production of interleukin-6. These results indicate that the fusion protein is useful for characterizing the physiological roles of the TAK1-TAB1 complex.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adaptor Proteins, Signal Transducing,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-6,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-8,
http://linkedlifedata.com/resource/pubmed/chemical/Intracellular Signaling Peptides...,
http://linkedlifedata.com/resource/pubmed/chemical/JNK Mitogen-Activated Protein...,
http://linkedlifedata.com/resource/pubmed/chemical/MAP Kinase Kinase Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/MAP kinase kinase kinase 7,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/TAB1 protein, MAPKKK activator...,
http://linkedlifedata.com/resource/pubmed/chemical/TAB1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factor AP-1,
http://linkedlifedata.com/resource/pubmed/chemical/p38 Mitogen-Activated Protein...
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
11
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pubmed:volume |
297
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1277-81
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pubmed:dateRevised |
2011-11-2
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pubmed:meshHeading |
pubmed-meshheading:12372426-Adaptor Proteins, Signal Transducing,
pubmed-meshheading:12372426-Binding Sites,
pubmed-meshheading:12372426-Blotting, Western,
pubmed-meshheading:12372426-Carrier Proteins,
pubmed-meshheading:12372426-DNA, Complementary,
pubmed-meshheading:12372426-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:12372426-HeLa Cells,
pubmed-meshheading:12372426-Humans,
pubmed-meshheading:12372426-Interleukin-6,
pubmed-meshheading:12372426-Interleukin-8,
pubmed-meshheading:12372426-Intracellular Signaling Peptides and Proteins,
pubmed-meshheading:12372426-JNK Mitogen-Activated Protein Kinases,
pubmed-meshheading:12372426-MAP Kinase Kinase Kinases,
pubmed-meshheading:12372426-MAP Kinase Signaling System,
pubmed-meshheading:12372426-Mitogen-Activated Protein Kinases,
pubmed-meshheading:12372426-Models, Genetic,
pubmed-meshheading:12372426-Mutation,
pubmed-meshheading:12372426-NF-kappa B,
pubmed-meshheading:12372426-Precipitin Tests,
pubmed-meshheading:12372426-Protein Binding,
pubmed-meshheading:12372426-Protein Structure, Tertiary,
pubmed-meshheading:12372426-Recombinant Fusion Proteins,
pubmed-meshheading:12372426-Transcription Factor AP-1,
pubmed-meshheading:12372426-Transfection,
pubmed-meshheading:12372426-p38 Mitogen-Activated Protein Kinases
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pubmed:year |
2002
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pubmed:articleTitle |
TAK1-TAB1 fusion protein: a novel constitutively active mitogen-activated protein kinase kinase kinase that stimulates AP-1 and NF-kappaB signaling pathways.
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pubmed:affiliation |
Discovery Research Laboratory, Tanabe Seiyaku Co., Ltd., 16-89 Kashima 3-chome, Yodogawa-ku, 532-8505, Osaka, Japan.
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pubmed:publicationType |
Journal Article
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