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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
2002-10-9
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pubmed:abstractText |
The introduction of several sets of amino acid substitutions into the region around a substrate-binding site of a medium-chain (all-E) prenyl diphosphate synthase, hexaprenyl diphosphate synthase from a thermoacidophilic archaeon Sulfolobus solfataricus, to mimic the product determination mechanisms of various kinds of short-chain enzymes revealed that the structure around the region of the medium-chain enzyme resembles those of eukaryotic farnesyl diphosphate synthases but not those of the other short-chain enzymes, reflecting the evolutional relationships among these enzymes.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
11
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pubmed:volume |
297
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1096-101
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:12372398-Alkyl and Aryl Transferases,
pubmed-meshheading:12372398-Amino Acid Sequence,
pubmed-meshheading:12372398-Binding Sites,
pubmed-meshheading:12372398-Chromatography, Thin Layer,
pubmed-meshheading:12372398-Dimerization,
pubmed-meshheading:12372398-Dimethylallyltranstransferase,
pubmed-meshheading:12372398-Escherichia coli,
pubmed-meshheading:12372398-Evolution, Molecular,
pubmed-meshheading:12372398-Molecular Sequence Data,
pubmed-meshheading:12372398-Mutagenesis, Site-Directed,
pubmed-meshheading:12372398-Mutation,
pubmed-meshheading:12372398-Plasmids,
pubmed-meshheading:12372398-Sequence Homology, Amino Acid,
pubmed-meshheading:12372398-Substrate Specificity,
pubmed-meshheading:12372398-Sulfolobus,
pubmed-meshheading:12372398-Temperature
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pubmed:year |
2002
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pubmed:articleTitle |
Change of product specificity of hexaprenyl diphosphate synthase from Sulfolobus solfataricus by introducing mimetic mutations.
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pubmed:affiliation |
Department of Biochemistry and Engineering, Graduate School of Engineering, Tohoku University, Aoba-yama 07, Sendai-shi, 980-8579, Miyagi-ken, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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