Linked Life Data

12370246

Source:http://linkedlifedata.com/resource/pubmed/id/12370246

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2002-10-15
pubmed:abstractText
Transcytosis is used alone (e.g., hepatoma HepG2 cells) or in combination with a direct pathway from the Golgi (e.g., epithelial MDCK cells) as an indirect route for targeting proteins to the apical surface. The raft-associated MAL protein is an essential element of the machinery for the direct route in MDCK cells. Herein, we present the functional characterization of MAL2, a member of the MAL protein family, in polarized HepG2 cells. MAL2 resided selectively in rafts and is predominantly distributed in a compartment localized beneath the subapical F-actin cytoskeleton. MAL2 greatly colocalized in subapical endosome structures with transcytosing molecules en route to the apical surface. Depletion of endogenous MAL2 drastically blocked transcytotic transport of exogenous polymeric immunoglobulin receptor and endogenous glycosylphosphatidylinositol-anchored protein CD59 to the apical membrane. MAL2 depletion did not affect the internalization of these molecules but produced their accumulation in perinuclear endosome elements that were accessible to transferrin. Normal transcytosis persisted in cells that expressed exogenous MAL2 designed to resist the depletion treatment. MAL2 is therefore essential for transcytosis in HepG2 cells.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/12370246-10189374, http://linkedlifedata.com/resource/pubmed/commentcorrection/12370246-10339572, http://linkedlifedata.com/resource/pubmed/commentcorrection/12370246-10848627, http://linkedlifedata.com/resource/pubmed/commentcorrection/12370246-10873817, http://linkedlifedata.com/resource/pubmed/commentcorrection/12370246-11549320, http://linkedlifedata.com/resource/pubmed/commentcorrection/12370246-11673461, http://linkedlifedata.com/resource/pubmed/commentcorrection/12370246-11809835, http://linkedlifedata.com/resource/pubmed/commentcorrection/12370246-1447295, http://linkedlifedata.com/resource/pubmed/commentcorrection/12370246-1531449, http://linkedlifedata.com/resource/pubmed/commentcorrection/12370246-2170116, http://linkedlifedata.com/resource/pubmed/commentcorrection/12370246-2196994, http://linkedlifedata.com/resource/pubmed/commentcorrection/12370246-3494249, http://linkedlifedata.com/resource/pubmed/commentcorrection/12370246-3524859, http://linkedlifedata.com/resource/pubmed/commentcorrection/12370246-8138576, http://linkedlifedata.com/resource/pubmed/commentcorrection/12370246-8390592, http://linkedlifedata.com/resource/pubmed/commentcorrection/12370246-9126323, http://linkedlifedata.com/resource/pubmed/commentcorrection/12370246-9658181
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0021-9525
pubmed:author
pubmed:issnType
Print
pubmed:day
14
pubmed:volume
159
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
37-44
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2002
pubmed:articleTitle
MAL2, a novel raft protein of the MAL family, is an essential component of the machinery for transcytosis in hepatoma HepG2 cells.
pubmed:affiliation
Centro de Biología Molecular "Severo Ochoa", Centro Nacional de Biotecnología, Universidad Autónoma de Madrid and Consejo Superior de Investigaciones Científicas, Cantoblanco, 28049 Madrid, Spain.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't