Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
2002-10-7
pubmed:abstractText
Phosphatidylinositol (PI) metabolism plays a central role in signaling pathways in both animals and higher plants. Stomatal guard cells have been reported to contain PI 3-phosphate (PI3P) and PI 4-phosphate (PI4P), the products of PI 3-kinase (PI3K) and PI 4-kinase (PI4K) activities. In this study, we tested the roles of PI3P and PI4P in stomatal movements. Both wortmannin (WM) and LY294002 inhibited PI3K and PI4K activities in guard cells and promoted stomatal opening induced by white light or the circadian clock. WM and LY294002 also inhibited stomatal closing induced by abscisic acid (ABA). Furthermore, overexpression in guard cells of GFP:EBD (green fluorescent protein:endosome binding domain of human EEA1) or GFP:FAPP1PH (PI-four-P adaptor protein-1 pleckstrin homology domain), which bind to PI3P and PI4P, respectively, increased stomatal apertures under darkness and white light and partially inhibited stomatal closing induced by ABA. The reduction in ABA-induced stomatal closing with reduced levels of PI monophosphate seemed to be attributable, at least in part, to impaired Ca(2+) signaling, because WM and LY294002 inhibited ABA-induced cytosolic Ca(2+) increases in guard cells. These results suggest that PI3P and PI4P play an important role in the modulation of stomatal closing and that reductions in the levels of functional PI3P and PI4P enhance stomatal opening.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/12368494, http://linkedlifedata.com/resource/pubmed/commentcorrection/12368494-10026194, http://linkedlifedata.com/resource/pubmed/commentcorrection/12368494-10085060, http://linkedlifedata.com/resource/pubmed/commentcorrection/12368494-10209027, http://linkedlifedata.com/resource/pubmed/commentcorrection/12368494-10322451, http://linkedlifedata.com/resource/pubmed/commentcorrection/12368494-10333579, http://linkedlifedata.com/resource/pubmed/commentcorrection/12368494-10348740, http://linkedlifedata.com/resource/pubmed/commentcorrection/12368494-10482669, http://linkedlifedata.com/resource/pubmed/commentcorrection/12368494-10571859, http://linkedlifedata.com/resource/pubmed/commentcorrection/12368494-10744745, http://linkedlifedata.com/resource/pubmed/commentcorrection/12368494-10750900, http://linkedlifedata.com/resource/pubmed/commentcorrection/12368494-10781106, http://linkedlifedata.com/resource/pubmed/commentcorrection/12368494-10837261, http://linkedlifedata.com/resource/pubmed/commentcorrection/12368494-10838616, http://linkedlifedata.com/resource/pubmed/commentcorrection/12368494-10930462, http://linkedlifedata.com/resource/pubmed/commentcorrection/12368494-10963598, http://linkedlifedata.com/resource/pubmed/commentcorrection/12368494-10970851, http://linkedlifedata.com/resource/pubmed/commentcorrection/12368494-11001876, http://linkedlifedata.com/resource/pubmed/commentcorrection/12368494-11006340, http://linkedlifedata.com/resource/pubmed/commentcorrection/12368494-11226186, http://linkedlifedata.com/resource/pubmed/commentcorrection/12368494-11268200, http://linkedlifedata.com/resource/pubmed/commentcorrection/12368494-11340192, http://linkedlifedata.com/resource/pubmed/commentcorrection/12368494-11395417, http://linkedlifedata.com/resource/pubmed/commentcorrection/12368494-11429606, http://linkedlifedata.com/resource/pubmed/commentcorrection/12368494-11449048, http://linkedlifedata.com/resource/pubmed/commentcorrection/12368494-1312084, http://linkedlifedata.com/resource/pubmed/commentcorrection/12368494-1656463, http://linkedlifedata.com/resource/pubmed/commentcorrection/12368494-16664518, http://linkedlifedata.com/resource/pubmed/commentcorrection/12368494-2388697, http://linkedlifedata.com/resource/pubmed/commentcorrection/12368494-7479754, http://linkedlifedata.com/resource/pubmed/commentcorrection/12368494-7559754, http://linkedlifedata.com/resource/pubmed/commentcorrection/12368494-7937816, http://linkedlifedata.com/resource/pubmed/commentcorrection/12368494-7972072, http://linkedlifedata.com/resource/pubmed/commentcorrection/12368494-8387919, http://linkedlifedata.com/resource/pubmed/commentcorrection/12368494-9512420, http://linkedlifedata.com/resource/pubmed/commentcorrection/12368494-9512651, http://linkedlifedata.com/resource/pubmed/commentcorrection/12368494-9517004, http://linkedlifedata.com/resource/pubmed/commentcorrection/12368494-9724630, http://linkedlifedata.com/resource/pubmed/commentcorrection/12368494-9838049, http://linkedlifedata.com/resource/pubmed/commentcorrection/12368494-9838059, http://linkedlifedata.com/resource/pubmed/commentcorrection/12368494-9865696, http://linkedlifedata.com/resource/pubmed/commentcorrection/12368494-9990101
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/1-Phosphatidylinositol 4-Kinase, http://linkedlifedata.com/resource/pubmed/chemical/2-(4-morpholinyl)-8-phenyl-4H-1-benz..., http://linkedlifedata.com/resource/pubmed/chemical/Abscisic Acid, http://linkedlifedata.com/resource/pubmed/chemical/Androstadienes, http://linkedlifedata.com/resource/pubmed/chemical/Blood Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Calcium, http://linkedlifedata.com/resource/pubmed/chemical/Chromones, http://linkedlifedata.com/resource/pubmed/chemical/Green Fluorescent Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Luminescent Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Morpholines, http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylinositol 3-Kinases, http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylinositol Phosphates, http://linkedlifedata.com/resource/pubmed/chemical/Phosphoproteins, http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins, http://linkedlifedata.com/resource/pubmed/chemical/phosphatidylinositol 3-phosphate, http://linkedlifedata.com/resource/pubmed/chemical/phosphatidylinositol 4-phosphate, http://linkedlifedata.com/resource/pubmed/chemical/platelet protein P47, http://linkedlifedata.com/resource/pubmed/chemical/wortmannin
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1040-4651
pubmed:author
pubmed:issnType
Print
pubmed:volume
14
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2399-412
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
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