Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2002-10-7
pubmed:abstractText
The discovery of the genes for nephrin and podocin, which are mutated in two types of congenital nephrotic syndrome, was pivotal in establishing the podocyte as the central component of the glomerular filtration barrier. In vivo the proteins have been localized to the podocyte slit diaphragm, and there is recent evidence for interaction between the two via the adapter molecule CD2AP. We describe in a human podocyte cell line, the subcellular distribution of nephrin, podocins, and CD2AP and their functional interaction with the cytoskeleton. In addition to membrane expression, nephrin and podocin were detected intracellularly in a filamentous pattern. Double immunolabeling and depolymerization studies showed that nephrin and podocin partially co-localize with actin, most strikingly seen protruding from the tips of actin filaments, and are dependent on intact actin polymers for their intracellular distribution. Treatment of differentiated podocytes with puromycin aminonucleoside, an agent that causes foot process effacement in vivo, disrupted actin and nephrin simultaneously, with loss of cell surface localization. We demonstrate an intimate relationship between nephrin podocin and filamentous actin, and reason that disruption of nephrin/podocin could be a final common pathway leading to foot process effacement in proteinuric diseases.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/12368218-10393930, http://linkedlifedata.com/resource/pubmed/commentcorrection/12368218-10504499, http://linkedlifedata.com/resource/pubmed/commentcorrection/12368218-10514378, http://linkedlifedata.com/resource/pubmed/commentcorrection/12368218-10550324, http://linkedlifedata.com/resource/pubmed/commentcorrection/12368218-10616834, http://linkedlifedata.com/resource/pubmed/commentcorrection/12368218-10742096, http://linkedlifedata.com/resource/pubmed/commentcorrection/12368218-10792613, http://linkedlifedata.com/resource/pubmed/commentcorrection/12368218-10972661, http://linkedlifedata.com/resource/pubmed/commentcorrection/12368218-11007821, http://linkedlifedata.com/resource/pubmed/commentcorrection/12368218-11106563, http://linkedlifedata.com/resource/pubmed/commentcorrection/12368218-11136707, http://linkedlifedata.com/resource/pubmed/commentcorrection/12368218-11159550, http://linkedlifedata.com/resource/pubmed/commentcorrection/12368218-11562357, http://linkedlifedata.com/resource/pubmed/commentcorrection/12368218-11733557, http://linkedlifedata.com/resource/pubmed/commentcorrection/12368218-11856766, http://linkedlifedata.com/resource/pubmed/commentcorrection/12368218-11961028, http://linkedlifedata.com/resource/pubmed/commentcorrection/12368218-3976843, http://linkedlifedata.com/resource/pubmed/commentcorrection/12368218-8142275, http://linkedlifedata.com/resource/pubmed/commentcorrection/12368218-8306891, http://linkedlifedata.com/resource/pubmed/commentcorrection/12368218-8546330, http://linkedlifedata.com/resource/pubmed/commentcorrection/12368218-9314539, http://linkedlifedata.com/resource/pubmed/commentcorrection/12368218-9344605, http://linkedlifedata.com/resource/pubmed/commentcorrection/12368218-9535550, http://linkedlifedata.com/resource/pubmed/commentcorrection/12368218-9660941, http://linkedlifedata.com/resource/pubmed/commentcorrection/12368218-9741631
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0002-9440
pubmed:author
pubmed:issnType
Print
pubmed:volume
161
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1459-66
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2002
pubmed:articleTitle
Co-localization of nephrin, podocin, and the actin cytoskeleton: evidence for a role in podocyte foot process formation.
pubmed:affiliation
Children's Unit, University of Bristol, Southmead Hospital, Bristol, United Kingdom. m.saleem@bristol.ac.uk
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't