Linked Life Data

12366846

Source:http://linkedlifedata.com/resource/pubmed/id/12366846

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2002-10-7
pubmed:abstractText
Bacterial attachment to solid matrices depends on adhesive molecules present on the cell surface. Here we establish a positive correlation between peptidoglycan (PG) breaks, rather than particular molecules, and biofilm-forming capacity in the Gram-positive bacterium Lactococcus lactis. The L. lactis acmA strain, which is defective in PG hydrolase, adhered less efficiently than the wild-type (wt) strain to different solid surfaces and was unable to form biofilms. These phenotypes were abolished by addition of lysozyme, a PG hydrolytic enzyme. Thus, the presence of PG breaks introduced by PG hydrolase, and not the AcmA protein itself, appears to be responsible for biofilm formation. Two different genetic screens confirmed the importance of PG breaks in L. lactis biofilm formation. Using the chain-forming ability of the acmA strain as a phenotypic indicator of PG integrity, we selected for insertional mutants generating short chains. Five independent mutants were all mapped to ponA, which encodes the PG synthesis enzyme PBP1A. Double acmA ponA mutants displayed increased adhesion and biofilm-forming capacity. Direct selection for strains with increased biofilm-forming capacity resulted in the isolation of another five mutations in ponA. Based on these results, we conclude that PG breaks are important for both adhesion and biofilm formation in L. lactis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0950-382X
pubmed:author
pubmed:issnType
Print
pubmed:volume
46
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
235-43
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed-meshheading:12366846-Bacterial Adhesion, pubmed-meshheading:12366846-Bacterial Proteins, pubmed-meshheading:12366846-Biofilms, pubmed-meshheading:12366846-Carrier Proteins, pubmed-meshheading:12366846-Cell Wall, pubmed-meshheading:12366846-Hexosyltransferases, pubmed-meshheading:12366846-In Situ Hybridization, Fluorescence, pubmed-meshheading:12366846-Lactococcus lactis, pubmed-meshheading:12366846-Microscopy, Electron, Scanning, pubmed-meshheading:12366846-Multienzyme Complexes, pubmed-meshheading:12366846-Muramoylpentapeptide Carboxypeptidase, pubmed-meshheading:12366846-Mutation, pubmed-meshheading:12366846-N-Acetylmuramoyl-L-alanine Amidase, pubmed-meshheading:12366846-Penicillin-Binding Proteins, pubmed-meshheading:12366846-Peptidoglycan, pubmed-meshheading:12366846-Peptidyl Transferases
pubmed:year
2002
pubmed:articleTitle
Positive role of peptidoglycan breaks in lactococcal biofilm formation.
pubmed:affiliation
Unité de Recherches Laitières et de Génétique Appliquée, Institut National de la Recherche Agronomique, Domaine de Vilvert, 78352 Jouy-en-Josas cedex, France.
pubmed:publicationType
Journal Article