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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2002-10-3
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pubmed:abstractText |
Centrosome duplication and separation are linked inextricably to certain cell cycle events, in particular activation of cyclin-dependent kinases (CDKs). However, relatively few CDK targets driving these events have been uncovered. Here, we have performed a screen for CDK substrates and have isolated a target, CP110, which is phosphorylated by CDKs in vitro and in vivo. Human CP110 localizes to centrosomes. Its expression is strongly induced at the G1-to-S phase transition, coincident with the initiation of centrosome duplication. RNAi-mediated depletion of CP110 indicates that this protein plays an essential role in centrosome duplication. Long-term disruption of CP110 phosphorylation leads to unscheduled centrosome separation and overt polyploidy. Our data suggest that CP110 is a physiological centrosomal CDK target that promotes centrosome duplication, and its deregulation may contribute to genomic instability.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
1534-5807
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
3
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
339-50
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:12361598-Amino Acid Motifs,
pubmed-meshheading:12361598-Amino Acid Sequence,
pubmed-meshheading:12361598-Cell Cycle Proteins,
pubmed-meshheading:12361598-Centrosome,
pubmed-meshheading:12361598-Cloning, Molecular,
pubmed-meshheading:12361598-Cyclin-Dependent Kinases,
pubmed-meshheading:12361598-DNA, Complementary,
pubmed-meshheading:12361598-G1 Phase,
pubmed-meshheading:12361598-Gene Expression Regulation,
pubmed-meshheading:12361598-HeLa Cells,
pubmed-meshheading:12361598-Humans,
pubmed-meshheading:12361598-In Situ Hybridization, Fluorescence,
pubmed-meshheading:12361598-Microtubule-Associated Proteins,
pubmed-meshheading:12361598-Molecular Sequence Data,
pubmed-meshheading:12361598-Mutagenesis, Site-Directed,
pubmed-meshheading:12361598-Phosphoproteins,
pubmed-meshheading:12361598-Phosphorylation,
pubmed-meshheading:12361598-Polyploidy,
pubmed-meshheading:12361598-RNA, Small Interfering,
pubmed-meshheading:12361598-Recombinant Proteins,
pubmed-meshheading:12361598-S Phase,
pubmed-meshheading:12361598-Sensitivity and Specificity,
pubmed-meshheading:12361598-Sequence Alignment,
pubmed-meshheading:12361598-Substrate Specificity,
pubmed-meshheading:12361598-Tumor Cells, Cultured
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pubmed:year |
2002
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pubmed:articleTitle |
CP110, a cell cycle-dependent CDK substrate, regulates centrosome duplication in human cells.
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pubmed:affiliation |
Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Avenue, Cambridge, MA 02138, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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