Source:http://linkedlifedata.com/resource/pubmed/id/12360106
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
7
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pubmed:dateCreated |
2002-10-2
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pubmed:abstractText |
Dihydropyrimidine dehydrogenase (DPD) is the initial and rate-limiting enzyme in the catabolism of 5-fluorouracil (5FU) and a DPD deficiency is increasingly being recognized as an important pharmacogenetic factor in the aetiology of severe 5FU-associated toxicity. In this study, we evaluated the DPD activity and the prevalence of the common splice site mutation IVS14 + 1G>A in tumour patients suffering from severe grade 3-4 toxicity after the administration of 5FU. DPD activity was measured with a radiochemical assay and screening for the presence of the IVS14 + 1G>A mutation was performed by restriction fragment length polymorphism. A decreased DPD activity could be detected in peripheral blood mononuclear cells in 60% of the cases. Furthermore, a high prevalence of the IVS14 + 1G>A mutation was noted as 28% of all patients were heterozygous or homozygous for this mutation. In patients with a low DPD activity, 42% were heterozygous and one patient (3%) was homozygous for the IVS14 + 1G>A mutation. In contrast, the IVS14 + 1G>A mutation could be detected in only one out of 24 (4%) patients with a normal DPD activity. Our study demonstrates that a DPD deficiency is the major determinant of 5FU-associated toxicity. The apparently high prevalence of the IVS14 + 1G>A mutation warrants genetic screening for this mutation in cancer patients before the administration of 5FU.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antimetabolites, Antineoplastic,
http://linkedlifedata.com/resource/pubmed/chemical/Dihydrouracil Dehydrogenase (NADP),
http://linkedlifedata.com/resource/pubmed/chemical/Fluorouracil,
http://linkedlifedata.com/resource/pubmed/chemical/Oxidoreductases
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0960-314X
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
12
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
555-8
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:12360106-Adult,
pubmed-meshheading:12360106-Aged,
pubmed-meshheading:12360106-Alternative Splicing,
pubmed-meshheading:12360106-Antimetabolites, Antineoplastic,
pubmed-meshheading:12360106-Dihydrouracil Dehydrogenase (NADP),
pubmed-meshheading:12360106-Female,
pubmed-meshheading:12360106-Fluorouracil,
pubmed-meshheading:12360106-Granulocytes,
pubmed-meshheading:12360106-Heterozygote Detection,
pubmed-meshheading:12360106-Homozygote,
pubmed-meshheading:12360106-Humans,
pubmed-meshheading:12360106-Leukocytes, Mononuclear,
pubmed-meshheading:12360106-Male,
pubmed-meshheading:12360106-Middle Aged,
pubmed-meshheading:12360106-Neoplasms,
pubmed-meshheading:12360106-Oxidoreductases,
pubmed-meshheading:12360106-Polymorphism, Restriction Fragment Length
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pubmed:year |
2002
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pubmed:articleTitle |
High prevalence of the IVS14 + 1G>A mutation in the dihydropyrimidine dehydrogenase gene of patients with severe 5-fluorouracil-associated toxicity.
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pubmed:affiliation |
Academic Medical Center, University of Amsterdam, Emma Children's Hospital and Department of Clinical Chemistry, Amsterdam, The Netherlands. a.b.vanKuilenburg@amc.uva.nl
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pubmed:publicationType |
Journal Article
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