Linked Life Data

12359442

Source:http://linkedlifedata.com/resource/pubmed/id/12359442

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2002-10-2
pubmed:abstractText
Monocyte-derived dendritic cells (DCs) from adult T cell leukemia are impaired in taking up exogenous antigens. To overcome this impairment, we fused unpulsed DCs to human T-lymphotropic virus type I (HTLV-I)-infected CD4(+) T cells (fusion DC-T cells). The efficiency of fusion was 50% and the fusion cells expressed higher HLA-ABC and CD86 Ags than HTLV-I-infected DCs. The fusion DC-T cells stimulated autologous CD4(+) and CD8(+) T cells, but DCs fused to itself or PHA-blasts did not stimulate any subsets. The functionally highest fusion DC-T cells was obtained when a DC and HTLV-I-infected T cells were fused at a ratio of 3:1. Expression of HTLV-Igag Ag on CD4(+) T cells was up-regulated when infected in the presence of 8-azaguanine, and these fusion DC-T cells were quite efficient in induction of higher CD8(+) T cell response. The results suggest that fusion DC-T cells produce functionally competent Ag-presenting cells and may be a likely candidate for immunotherapeutic use.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0042-6822
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
301
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
13-20
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
2002
pubmed:articleTitle
Fusion of mature dendritic cells and human T-lymphotropic virus type I infected T cells: its efficiency as an antigen-presenting cell.
pubmed:affiliation
Department of Microbiology, Leprosy Research Center, National Institute of Infectious Diseases, Tokyo, 189-0002, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't