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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2002-10-2
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pubmed:abstractText |
Chicken prepro-orexin cDNA has been cloned, sequenced and characterized. The predicted amino acid sequence of chicken prepro-orexin cDNA revealed that orexin-A and -B are highly conserved among vertebrate species. In situ hybridization and immunohistochemistry localized orexin-positive cell bodies in the periventricular hypothalamic nucleus extending into the lateral hypothalamic area. Comparisons of orexin gene expression in the brains of 24-h-fasted and ad libitum-fed chickens were made using semi-quantitative RT-PCR. No significant differences in orexin mRNA expression were observed.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0006-3002
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
27
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pubmed:volume |
1577
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
476-80
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:12359340-Amino Acid Sequence,
pubmed-meshheading:12359340-Animals,
pubmed-meshheading:12359340-Base Sequence,
pubmed-meshheading:12359340-Chickens,
pubmed-meshheading:12359340-Cloning, Molecular,
pubmed-meshheading:12359340-DNA, Complementary,
pubmed-meshheading:12359340-Evolution, Molecular,
pubmed-meshheading:12359340-Hypothalamus,
pubmed-meshheading:12359340-Immunohistochemistry,
pubmed-meshheading:12359340-In Situ Hybridization,
pubmed-meshheading:12359340-Intracellular Signaling Peptides and Proteins,
pubmed-meshheading:12359340-Molecular Sequence Data,
pubmed-meshheading:12359340-Neuropeptides,
pubmed-meshheading:12359340-Prosencephalon,
pubmed-meshheading:12359340-Protein Precursors,
pubmed-meshheading:12359340-RNA,
pubmed-meshheading:12359340-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:12359340-Sequence Alignment
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pubmed:year |
2002
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pubmed:articleTitle |
Molecular cloning of chicken prepro-orexin cDNA and preferential expression in the chicken hypothalamus.
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pubmed:affiliation |
Center for Molecular Biology and Genetics, Mie University, Tsu, Japan. ohkubo@ag.kagawa-u.ac.jp
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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