| pubmed-article:12359069 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12359069 | lifeskim:mentions | umls-concept:C1512977 | lld:lifeskim |
| pubmed-article:12359069 | lifeskim:mentions | umls-concept:C0012854 | lld:lifeskim |
| pubmed-article:12359069 | lifeskim:mentions | umls-concept:C0049308 | lld:lifeskim |
| pubmed-article:12359069 | lifeskim:mentions | umls-concept:C0030011 | lld:lifeskim |
| pubmed-article:12359069 | lifeskim:mentions | umls-concept:C1514468 | lld:lifeskim |
| pubmed-article:12359069 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:12359069 | pubmed:dateCreated | 2002-10-2 | lld:pubmed |
| pubmed-article:12359069 | pubmed:abstractText | To examine the mutagenicity of 5-formylcytosine (5-fC), an oxidation product of 5-methylcytosine (5-mC), 5-fC was incorporated into predetermined sites of double-stranded shuttle vectors. The nucleotide sequences in which the modified base was incorporated were 5'-AFGCGT-3' and 5'-ACGFGT-3' (F represents 5-fC), the recognition site for the restriction enzyme MluI (5'-ACGCGT-3'). 5-fC was incorporated into the template strand of either the leading or lagging strand of DNA replication. The modified DNAs were transfected into simian COS-7 cells, and the DNAs replicated in the cells were recovered and analyzed after a second transfection into Escherichia coli. 5-fC weakly blocked DNA replication in mammalian cells. The 5-fC residues were mutagenic, with mutation frequencies in double-stranded vectors of 0.03-0.28%. The mutation spectrum of 5-fC was broad, and included targeted (5-fC-->G, 5-fC-->A, and 5-fC-->T) and untargeted mutations. These results suggest that the oxidation of 5-mC results in mutations at and around the modified sites. | lld:pubmed |
| pubmed-article:12359069 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12359069 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12359069 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:12359069 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12359069 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12359069 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12359069 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12359069 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12359069 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:12359069 | pubmed:issn | 0021-924X | lld:pubmed |
| pubmed-article:12359069 | pubmed:author | pubmed-author:KamiyaHiroyuk... | lld:pubmed |
| pubmed-article:12359069 | pubmed:author | pubmed-author:KarinoNaokoN | lld:pubmed |
| pubmed-article:12359069 | pubmed:author | pubmed-author:UenoYoshihito... | lld:pubmed |
| pubmed-article:12359069 | pubmed:author | pubmed-author:MatsudaAkiraA | lld:pubmed |
| pubmed-article:12359069 | pubmed:author | pubmed-author:HarashimaHide... | lld:pubmed |
| pubmed-article:12359069 | pubmed:author | pubmed-author:TsuchiyaHiroy... | lld:pubmed |
| pubmed-article:12359069 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12359069 | pubmed:volume | 132 | lld:pubmed |
| pubmed-article:12359069 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12359069 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12359069 | pubmed:pagination | 551-5 | lld:pubmed |
| pubmed-article:12359069 | pubmed:dateRevised | 2007-12-19 | lld:pubmed |
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| pubmed-article:12359069 | pubmed:meshHeading | pubmed-meshheading:12359069... | lld:pubmed |
| pubmed-article:12359069 | pubmed:meshHeading | pubmed-meshheading:12359069... | lld:pubmed |
| pubmed-article:12359069 | pubmed:meshHeading | pubmed-meshheading:12359069... | lld:pubmed |
| pubmed-article:12359069 | pubmed:meshHeading | pubmed-meshheading:12359069... | lld:pubmed |
| pubmed-article:12359069 | pubmed:meshHeading | pubmed-meshheading:12359069... | lld:pubmed |
| pubmed-article:12359069 | pubmed:year | 2002 | lld:pubmed |
| pubmed-article:12359069 | pubmed:articleTitle | Mutagenicity of 5-formylcytosine, an oxidation product of 5-methylcytosine, in DNA in mammalian cells. | lld:pubmed |
| pubmed-article:12359069 | pubmed:affiliation | Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita-12, Nishi-6, Kita-ku, Sapporo 060-0812, Japan. hirokam@pharm.hokudai.ac.jp | lld:pubmed |
| pubmed-article:12359069 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:12359069 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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