Linked Life Data

12356265

Source:http://linkedlifedata.com/resource/pubmed/id/12356265

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2002-10-1
pubmed:abstractText
All vertebrates have directional asymmetries in the organization of their internal organs. In jawed vertebrates, development of asymmetry is controlled by a conserved molecular pathway that includes Pitx2, which is expressed by lateral plate mesoderm cells on the left side of the embryo. Pitx2 is a member of the Pitx homeobox gene family, the expression of which also marks stomodeal ectoderm and the adenohypophysis. Here we report the characterization of Pitx genes from Branchiostoma floridae (an amphioxus) and Ciona intestinalis (a urochordate), representatives of two basal chordate lineages and successively deeper outgroups to the vertebrates. Expression of B. floridae Pitx is similar to that reported from B. belcheri, a different amphioxus species. Expression of the Ciona Pitx ortholog in the embryonic primordial pharynx and adult neural complex leads us to propose the Ciona primordial pharynx and ciliated funnel are homologous to the adenohypophyseal placode and adenohypophysis, respectively. Additionally, in both species we identify asymmetrical left-sided expression of Pitx genes during embryonic development. This shows that asymmetrical Pitx gene expression, and by inference directional asymmetry, evolved before the radiation of living chordates and should be considered a chordate character.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1520-541X
pubmed:author
pubmed:issnType
Print
pubmed:volume
4
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
354-65
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:12356265-Amino Acid Sequence, pubmed-meshheading:12356265-Animals, pubmed-meshheading:12356265-Base Sequence, pubmed-meshheading:12356265-Chordata, Nonvertebrate, pubmed-meshheading:12356265-Ciona intestinalis, pubmed-meshheading:12356265-Cloning, Molecular, pubmed-meshheading:12356265-DNA Primers, pubmed-meshheading:12356265-Evolution, Molecular, pubmed-meshheading:12356265-Genes, Homeobox, pubmed-meshheading:12356265-Homeodomain Proteins, pubmed-meshheading:12356265-In Situ Hybridization, pubmed-meshheading:12356265-Molecular Sequence Data, pubmed-meshheading:12356265-Nuclear Proteins, pubmed-meshheading:12356265-Phylogeny, pubmed-meshheading:12356265-Pituitary Gland, Anterior, pubmed-meshheading:12356265-Sequence Homology, Amino Acid, pubmed-meshheading:12356265-Transcription Factors
pubmed:articleTitle
Pitx homeobox genes in Ciona and amphioxus show left-right asymmetry is a conserved chordate character and define the ascidian adenohypophysis.
pubmed:affiliation
School of Animal and Microbial Sciences, University of Reading, Whiteknights, UK.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't