Linked Life Data

12356262

Source:http://linkedlifedata.com/resource/pubmed/id/12356262

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2002-10-1
pubmed:abstractText
SUMMARY Caenorhabditis elegans possesses two Notch-like receptors, LIN-12 and GLP-1, which have both overlapping and individual biological functions. We examined the lin-12 and glp-1 genes in closely related nematodes to learn about their evolution. Here we report molecular and functional analyses of lin-12 orthologs from two related nematodes, C. briggsae (Cb) and C. remanei (Cr). In addition, we compare these lin-12 findings with similar studies of Cb-glp-1 and Cr-glp-1 orthologs. Cb-LIN-12 and Cr-LIN-12 retain the same number and order of motifs as Ce-LIN-12. Intriguingly, we find that LIN-12 conservation differs from that of GLP-1 in two respects. First, individual motifs are conserved to a different degree for the two receptors. For example, the transmembrane domain is 16-32% identical among LIN-12 orthologs but 65-70% identical among GLP-1 orthologs. Second, certain amino acids are conserved in a receptor-specific manner, a phenomenon most prevalent in the CC-linker. We suggest that LIN-12 and GLP-1 have been molded by selective constraints that are receptor specific and that the two proteins may not be entirely interchangeable. To analyze the functions of the lin-12 orthologs, we used RNA-mediated interference (RNAi). Cb-lin-12(RNAi) or Cr-lin-12(RNAi) progeny are nearly 100% Lag, a larval lethality typical of C. elegans lin-12 glp-1 double mutants, but not the primary defect observed in Ce-lin-12 null mutants or Ce-lin-12(RNAi). Therefore, LIN-12 functions are similar, but not identical, among the Caenorhabditis species. We suggest that ancestral functions may have been divided between LIN-12 and GLP-1 receptors in a process contributing to the retention of both genes after gene duplication (i.e., subfunctionalization).
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1520-541X
pubmed:author
pubmed:issnType
Print
pubmed:volume
4
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
319-33
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed-meshheading:12356262-Amino Acid Sequence, pubmed-meshheading:12356262-Animals, pubmed-meshheading:12356262-Base Sequence, pubmed-meshheading:12356262-Biological Evolution, pubmed-meshheading:12356262-Blotting, Southern, pubmed-meshheading:12356262-Caenorhabditis elegans, pubmed-meshheading:12356262-Caenorhabditis elegans Proteins, pubmed-meshheading:12356262-Cloning, Molecular, pubmed-meshheading:12356262-DNA Primers, pubmed-meshheading:12356262-Gene Duplication, pubmed-meshheading:12356262-Helminth Proteins, pubmed-meshheading:12356262-Membrane Proteins, pubmed-meshheading:12356262-Molecular Sequence Data, pubmed-meshheading:12356262-Phenotype, pubmed-meshheading:12356262-Phylogeny, pubmed-meshheading:12356262-RNA, Messenger, pubmed-meshheading:12356262-Sequence Homology, Amino Acid
pubmed:articleTitle
Evolution of discrete Notch-like receptors from a distant gene duplication in Caenorhabditis.
pubmed:affiliation
Department of Biochemistry, University of Wisconsin-Madison, 53706, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't