Linked Life Data

12355555

Source:http://linkedlifedata.com/resource/pubmed/id/12355555

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2002-9-30
pubmed:abstractText
Heavy-ion radiation accounts for the major component of absorbed cosmic radiation and is thus regarded as a significant risk during long-term manned space missions. To evaluate the genetic damage induced by heavy particle radiation, gpt delta transgenic mice were exposed to carbon particle irradiation and the induced mutations were compared with those induced by reference radiations, i.e., X-rays and gamma-rays. In the transgenic mouse model, deletions and point mutations were individually identified as Spi(-) and gpt mutations, respectively. Two days after 10 Gy of whole-body irradiation, the mutant frequencies (MFs) of Spi(-) and gpt were determined. Carbon particle irradiation significantly increased Spi(-) MF in the liver, spleen, and kidney but not in the testis, suggesting an organ-specific induction of mutations by heavy-ion irradiation. In the liver, the potency of inducing Spi(-) mutation was highest for carbon particles (3.3-fold increase) followed by X-rays (2.1-fold increase) and gamma-rays (1.3-fold increase), while the potency of inducing gpt mutations was highest for gamma-rays (3.3-fold increase) followed by X-rays (2.1-fold increase) and carbon particles (1.6-fold increase). DNA sequence analysis revealed that carbon particles induced deletions that were mainly more than 1,000 base pairs in size, whereas gamma-rays induced deletions of less than 100 base pairs and base substitutions. X-rays induced various-sized deletions and base substitutions. These results suggest that heavy-ion beam irradiation is effective at inducing deletions via DNA double-strand breaks but less effective than X-ray and gamma-ray irradiation at producing oxidative DNA damage by free radicals.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0893-6692
pubmed:author
pubmed:copyrightInfo
Copyright 2002 Wiley-Liss, Inc.
pubmed:issnType
Print
pubmed:volume
40
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
207-15
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:12355555-Aerospace Medicine, pubmed-meshheading:12355555-Animals, pubmed-meshheading:12355555-Bacterial Proteins, pubmed-meshheading:12355555-Carbon, pubmed-meshheading:12355555-DNA, pubmed-meshheading:12355555-DNA Damage, pubmed-meshheading:12355555-DNA Mutational Analysis, pubmed-meshheading:12355555-Escherichia coli Proteins, pubmed-meshheading:12355555-Free Radicals, pubmed-meshheading:12355555-Gamma Rays, pubmed-meshheading:12355555-Heavy Ions, pubmed-meshheading:12355555-Homozygote, pubmed-meshheading:12355555-Kidney, pubmed-meshheading:12355555-Liver, pubmed-meshheading:12355555-Male, pubmed-meshheading:12355555-Mice, pubmed-meshheading:12355555-Mice, Transgenic, pubmed-meshheading:12355555-Mutation, pubmed-meshheading:12355555-Oxygen, pubmed-meshheading:12355555-Pentosyltransferases, pubmed-meshheading:12355555-Proteins, pubmed-meshheading:12355555-Spleen, pubmed-meshheading:12355555-Testis, pubmed-meshheading:12355555-Time Factors, pubmed-meshheading:12355555-Tissue Distribution, pubmed-meshheading:12355555-X-Rays
pubmed:year
2002
pubmed:articleTitle
Heavy-ion-induced mutations in the gpt delta transgenic mouse: comparison of mutation spectra induced by heavy-ion, X-ray, and gamma-ray radiation.
pubmed:affiliation
Division of Genetics and Mutagenesis, National Institute of Health Sciences, Tokyo, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't