Linked Life Data

12355460

Source:http://linkedlifedata.com/resource/pubmed/id/12355460

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2002-9-30
pubmed:abstractText
Batch kinetic and inhibition studies were performed for the aerobic cometabolism of 1,1,1-trichloroethane (1,1,1-TCA), 1,1-dichloroethylene (1,1-DCE), and 1,1-dichloroethane (1,1-DCA) by a butane-grown mixed culture. These chlorinated aliphatic hydrocarbons (CAHs) are often found together as cocontaminants in groundwater. The maximum degradation rates (k(max)) and half-saturation coefficients (K(s)) were determined in single compound kinetic tests. The highest k(max) was obtained for butane (2.6 micromol/mg TSS/h) followed by 1,1-DCE (1.3 micromol/mg TSS/h), 1,1-DCA (0.49 micromol/mg TSS/h), and 1,1,1-TCA (0.19 micromol/mg TSS/h), while the order of K(s) from the highest to lowest was 1,1-DCA (19 microM), butane (19 microM), 1,1,1-TCA (12 microM) and 1,1-DCE (1.5 microM). The inhibition types were determined using direct linear plots, while inhibition coefficients (K(ic) and K(iu)) were estimated by nonlinear least squares regression (NLSR) fits to the kinetic model of the identified inhibition type. Two different inhibition types were observed among the compounds. Competitive inhibition among CAHs was indicated from direct linear plots, and the CAHs also competitively inhibited butane utilization. 1,1-DCE was a stronger inhibitor than the other CAHs. Mixed inhibition of 1,1,1-TCA, 1,1-DCA, and 1,1-DCE transformations by butane was observed. Thus, both competitive and mixed inhibitions are important in cometabolism of CAHs by this butane culture. For competitive inhibition between CAHs, the ratio of the K(s) values was a reasonable indicator of competitive inhibition observed. Butane was a strong inhibitor of CAH transformation, having a much lower inhibition coefficient than the K(s) value of butane, while the CAHs were weak inhibitors of butane utilization. Model simulations of reactor systems where both the growth substrate and the CAHs are present indicate that reactor performance is significantly affected by inhibition type and inhibition coefficients. Thus, determining inhibition type and measuring inhibition coefficients is important in designing CAH treatment systems.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0006-3592
pubmed:author
pubmed:copyrightInfo
Copyright 2002 Wiley Periodicals, Inc.
pubmed:issnType
Print
pubmed:day
5
pubmed:volume
80
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
498-508
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:12355460-Bacteria, Aerobic, pubmed-meshheading:12355460-Biodegradation, Environmental, pubmed-meshheading:12355460-Bioreactors, pubmed-meshheading:12355460-Butanes, pubmed-meshheading:12355460-Dichloroethylenes, pubmed-meshheading:12355460-Enzyme Inhibitors, pubmed-meshheading:12355460-Ethylene Dichlorides, pubmed-meshheading:12355460-Hydrocarbons, Chlorinated, pubmed-meshheading:12355460-Kinetics, pubmed-meshheading:12355460-Models, Biological, pubmed-meshheading:12355460-Reproducibility of Results, pubmed-meshheading:12355460-Sensitivity and Specificity, pubmed-meshheading:12355460-Soil Microbiology, pubmed-meshheading:12355460-Soil Pollutants, pubmed-meshheading:12355460-Trichloroethanes, pubmed-meshheading:12355460-Water Pollutants, Chemical, pubmed-meshheading:12355460-Water Purification
pubmed:year
2002
pubmed:articleTitle
Kinetic and inhibition studies for the aerobic cometabolism of 1,1,1-trichloroethane, 1,1-dichloroethylene, and 1,1-dichloroethane by a butane-grown mixed culture.
pubmed:affiliation
Department of Civil, Construction, and Environmental Engineering, Oregon State University, Corvallis 97331-2302, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, Non-P.H.S., Evaluation Studies, Validation Studies