Linked Life Data

12354520

Source:http://linkedlifedata.com/resource/pubmed/id/12354520

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2-3
pubmed:dateCreated
2002-9-30
pubmed:abstractText
The expression of mRNA encoding interferon-gamma (IFN-gamma) and tumour necrosis factor-alpha (TNF-alpha) was studied, by reverse transcription-polymerase chain reaction and by in-situ hybridization with a non-radioactive digoxigenin-labelled probe, in formalin-fixed, paraffin wax-embedded lung tissue from pigs experimentally infected with a Korean isolate (North American genotype) of PRRSV. Both IFN-gamma and TNF-alpha mRNA were detected in the lung as early as 1 day post-inoculation (dpi) and up to 10 dpi. Hybridization signals for IFN-gamma and TNF-alpha were usually associated with inflammation, in particular with macrophages within alveolar septa. Expression of these cytokines was minimal in non-lesional lung of PRRSV-infected pigs and in normal lung from control pigs. The results suggest that IFN-gamma and TNF-alpha play a role in pulmonary defence against PRRSV.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0021-9975
pubmed:author
pubmed:issnType
Print
pubmed:volume
127
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
106-13
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:articleTitle
Expression of Interferon-gamma and tumour necrosis factor-alpha in pigs experimentally infected with Porcine Reproductive and Respiratory Syndrome Virus (PRRSV).
pubmed:affiliation
Department of Veterinary Pathology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, Suwon 441-744, Kyounggi-Do, Republic of Korea.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't