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rdf:type |
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lifeskim:mentions |
umls-concept:C0035820,
umls-concept:C0037083,
umls-concept:C0242609,
umls-concept:C0271510,
umls-concept:C1334536,
umls-concept:C1337107,
umls-concept:C1416958,
umls-concept:C1416959,
umls-concept:C1424628,
umls-concept:C1622917,
umls-concept:C1708842,
umls-concept:C1710082
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pubmed:issue |
5590
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pubmed:dateCreated |
2002-9-27
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pubmed:abstractText |
The spindle checkpoint delays sister chromatid separation until all chromosomes have undergone bipolar spindle attachment. Checkpoint failure may result in chromosome mis-segregation and may contribute to tumorigenesis. We showed that the human protein Hec1 was required for the recruitment of Mps1 kinase and Mad1/Mad2 complexes to kinetochores. Depletion of Hec1 impaired chromosome congression and caused persistent activation of the spindle checkpoint, indicating that high steady-state levels of Mad1/Mad2 complexes at kinetochores were not essential for checkpoint signaling. Simultaneous depletion of Hec1 and Mad2 caused catastrophic mitotic exit, making Hec1 an attractive target for the selective elimination of spindle checkpoint-deficient cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Calcium-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Cell Cycle Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Chromosomal Proteins, Non-Histone,
http://linkedlifedata.com/resource/pubmed/chemical/MAD2L1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Microtubule-Associated Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/NDC80 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Serine-Threonine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Small Interfering,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Untranslated,
http://linkedlifedata.com/resource/pubmed/chemical/Repressor Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/TTK protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/centromere protein E
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
1095-9203
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
27
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pubmed:volume |
297
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2267-70
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:12351790-Calcium-Binding Proteins,
pubmed-meshheading:12351790-Cell Cycle Proteins,
pubmed-meshheading:12351790-Chromosomal Proteins, Non-Histone,
pubmed-meshheading:12351790-Chromosomes, Human,
pubmed-meshheading:12351790-Gene Silencing,
pubmed-meshheading:12351790-HeLa Cells,
pubmed-meshheading:12351790-Humans,
pubmed-meshheading:12351790-Kinetochores,
pubmed-meshheading:12351790-Microtubule-Associated Proteins,
pubmed-meshheading:12351790-Microtubules,
pubmed-meshheading:12351790-Mitosis,
pubmed-meshheading:12351790-Mitotic Spindle Apparatus,
pubmed-meshheading:12351790-Nuclear Proteins,
pubmed-meshheading:12351790-Phenotype,
pubmed-meshheading:12351790-Protein Kinases,
pubmed-meshheading:12351790-Protein-Serine-Threonine Kinases,
pubmed-meshheading:12351790-RNA, Small Interfering,
pubmed-meshheading:12351790-RNA, Untranslated,
pubmed-meshheading:12351790-Repressor Proteins,
pubmed-meshheading:12351790-Signal Transduction,
pubmed-meshheading:12351790-Two-Hybrid System Techniques
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pubmed:year |
2002
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pubmed:articleTitle |
Role of Hec1 in spindle checkpoint signaling and kinetochore recruitment of Mad1/Mad2.
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pubmed:affiliation |
Department of Cell Biology, Max-Planck-Institute of Biochemistry, Am Klopferspitz 18a, D-82152 Martinsried, Germany.
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pubmed:publicationType |
Journal Article
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