Linked Life Data

12351723

Source:http://linkedlifedata.com/resource/pubmed/id/12351723

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
19
pubmed:dateCreated
2002-9-27
pubmed:abstractText
Organized synapse formation on to Purkinje cell (PC) dendrites by parallel fibers (PFs) and climbing fibers (CFs) is crucial for cerebellar function. In PCs lacking glutamate receptor delta2 (GluRdelta2), PF synapses are reduced in number, numerous free spines emerge, and multiple CF innervation persists to adulthood. In the present study, we conducted anterograde and immunohistochemical labelings to investigate how CFs innervate PC dendrites under weakened synaptogenesis by PFs. In the GluRdelta2 knock-out mouse, CFs were distributed in the molecular layer more closely to the pial surface compared with the wild-type mouse. Serial electron microscopy demonstrated that CFs in the knock-out mouse innervated all spines protruding from proximal dendrites of PCs, as did those in the wild-type mouse. In the knock-out mouse, however, CF innervation extended distally to spiny branchlets, where nearly half of the spines were free of innervation in contrast to complete synapse formation by PFs in the wild-type mouse. Furthermore, from the end point of innervation, CFs aberrantly jumped to form ectopic synapses on adjacent spiny branchlets, whose proximal portions were often innervated by different CFs. Without GluRdelta2, CFs are thus able to expand their territory along and beyond dendritic trees of the target PC, resulting in persistent surplus CFs by innervating the distal dendritic segment. We conclude that GluRdelta2 is essential to restrict CF innervation to the proximal dendritic segment, by which territorized innervation by PFs and CFs is properly structured and the formation of excess CF wiring to adjacent PCs is suppressed.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1529-2401
pubmed:author
pubmed:issnType
Electronic
pubmed:day
1
pubmed:volume
22
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
8487-503
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:12351723-Animals, pubmed-meshheading:12351723-Biotin, pubmed-meshheading:12351723-Carrier Proteins, pubmed-meshheading:12351723-Cerebellum, pubmed-meshheading:12351723-Dendrites, pubmed-meshheading:12351723-Dextrans, pubmed-meshheading:12351723-Immunohistochemistry, pubmed-meshheading:12351723-Iontophoresis, pubmed-meshheading:12351723-Membrane Transport Proteins, pubmed-meshheading:12351723-Mice, pubmed-meshheading:12351723-Mice, Knockout, pubmed-meshheading:12351723-Nerve Fibers, pubmed-meshheading:12351723-Nervous System Malformations, pubmed-meshheading:12351723-Neurons, Afferent, pubmed-meshheading:12351723-Olivary Nucleus, pubmed-meshheading:12351723-Purkinje Cells, pubmed-meshheading:12351723-Receptors, Glutamate, pubmed-meshheading:12351723-Synapses, pubmed-meshheading:12351723-Vesicular Glutamate Transport Protein 2, pubmed-meshheading:12351723-Vesicular Transport Proteins
pubmed:year
2002
pubmed:articleTitle
Distal extension of climbing fiber territory and multiple innervation caused by aberrant wiring to adjacent spiny branchlets in cerebellar Purkinje cells lacking glutamate receptor delta 2.
pubmed:affiliation
Department of Anatomy, Hokkaido University School of Medicine, Sapporo 060-8638, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't