Linked Life Data

12351211

Source:http://linkedlifedata.com/resource/pubmed/id/12351211

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2002-9-27
pubmed:abstractText
The monitoring of reactive oxygen species (ROS) levels in injured nervous tissue is critical for both studying the mechanism of secondary damage and evaluating the effectiveness of antioxidants. Flow cytometry is an excellent method to detect ROS in cultured cells and naturally suspended individual cells. However, its use in nervous tissue is limited due to the difficulties in obtaining single cells in suspension. We have developed a new method which minimizes the error during conventional dissociation. Specifically, we introduced a fixation step (with formaldehyde) between the dye loading and dissociation. As a result, the post-injury ROS signals detected by flow cytometry increase significantly when using hydroethidine as superoxide indicator. The injury-induced elevation of ROS obtained from this new method was also in better agreement with the two other standard ROS detection methods, fluorescence microscopy and lipid peroxidation assay. Furthermore, more pronounced decrease of ROS was found in this improved method in response to treatment with a superoxide scavenger, manganese(III)tetrakis(4-benzoic acid)porphyrin. Based on these observations, we suggest that the data obtained from the cells by this new method are more accurate than those from the classic cell dissociation method that dissociates cells directly from fresh tissues.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0165-0270
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
120
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
105-12
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2002
pubmed:articleTitle
Detection of reactive oxygen species by flow cytometry after spinal cord injury.
pubmed:affiliation
Department of Basic Medical Sciences, Center for Paralysis Research, School of Veterinary Medicine, Purdue University,West Lafayette, IN 47907, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't