Linked Life Data

12324366

Source:http://linkedlifedata.com/resource/pubmed/id/12324366

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
2002-9-26
pubmed:abstractText
We developed a double-staining procedure involving NanoOrange dye (Molecular Probes, Eugene, Oreg.) and membrane integrity stains (LIVE/DEAD BacLight kit; Molecular Probes) to show the morphological and membrane integrity changes of Campylobacter coli cells during growth. The conversion from a spiral to a coccoid morphology via intermediary forms and the membrane integrity changes of the C. coli cells can be detected with the double-staining procedure. Our data indicate that young or actively growing cells are mainly spiral shaped (green-stained cells), but older cells undergo a degenerative change to coccoid forms (red-stained cells). Club-shaped transition cell forms were observed with NanoOrange stain. Chlorinated drinking water affected the viability but not the morphology of C. coli cells.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/12324366-10049870, http://linkedlifedata.com/resource/pubmed/commentcorrection/12324366-10196767, http://linkedlifedata.com/resource/pubmed/commentcorrection/12324366-10508106, http://linkedlifedata.com/resource/pubmed/commentcorrection/12324366-10919832, http://linkedlifedata.com/resource/pubmed/commentcorrection/12324366-3767358, http://linkedlifedata.com/resource/pubmed/commentcorrection/12324366-4044525, http://linkedlifedata.com/resource/pubmed/commentcorrection/12324366-7703020, http://linkedlifedata.com/resource/pubmed/commentcorrection/12324366-7764089, http://linkedlifedata.com/resource/pubmed/commentcorrection/12324366-9572943, http://linkedlifedata.com/resource/pubmed/commentcorrection/12324366-9750311, http://linkedlifedata.com/resource/pubmed/commentcorrection/12324366-9835545
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0099-2240
pubmed:author
pubmed:issnType
Print
pubmed:volume
68
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
5151-4
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2002
pubmed:articleTitle
Double-staining method for differentiation of morphological changes and membrane integrity of Campylobacter coli cells.
pubmed:affiliation
Instituto de Hidrología y Medio Natural. Departamento de Biotecnología, Universidad Politécnica, 46022 Valencia, Spain.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't