|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
1-3
|
|
pubmed:dateCreated |
2002-9-25
|
|
pubmed:abstractText |
Hashimoto's encephalopathy (HE) is a rare autoimmune disease associated with Hashimoto's thyroiditis (HT). To identify the HE-related autoantigens, we developed a human brain proteome map using two-dimensional electrophoresis and applied it to the immuno-screening of brain proteins that react with autoantibodies in HE patients. After sequential MALDI-TOF-MASS analysis, immuno-positive spots of 48 kDa (pI 7.3-7.8) detected from HE patient sera were identified as a novel autoimmuno-antigen, alpha-enolase, harboring several modifications. Specific high reactivities against human alpha-enolase were significant in HE patients with excellent corticosteroid sensitivity, whereas the patients with fair or poor sensitivity to the corticosteroid treatment showed less reactivities than cut-off level. Although a few HT patients showed faint reactions to alpha-enolase, 95% of HT patients, patients with other neurological disorders, and healthy subjects tested were all negative. These results suggest that the detection of anti-alpha-enolase antibody is useful for defining HE-related pathology, and this proteomic strategy is a powerful method for identifying autoantigens of various central nervous system diseases with unknown autoimmune etiologies.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Sep
|
|
pubmed:issn |
0014-5793
|
|
pubmed:author |
pubmed-author:ArakiNorieN,
pubmed-author:ArakiTomohiroT,
pubmed-author:HoriuchiIzumiI,
pubmed-author:KiraJun-ichiJ,
pubmed-author:MizumotoKiyohisaK,
pubmed-author:MuraiHiroyukiH,
pubmed-author:OchiHirofumiH,
pubmed-author:OginoTomoakiT,
pubmed-author:OkamuraKenK,
pubmed-author:OsoegawaManabuM,
pubmed-author:SatoKaoriK,
pubmed-author:SayaHideyukiH,
pubmed-author:TodaTosifusaT,
pubmed-author:YamadaTakeshiT,
pubmed-author:YamashitaHirohumiH
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
25
|
|
pubmed:volume |
528
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
197-202
|
|
pubmed:dateRevised |
2006-11-15
|
|
pubmed:meshHeading |
pubmed-meshheading:12297304-Amino Acid Sequence,
pubmed-meshheading:12297304-Antibody Specificity,
pubmed-meshheading:12297304-Autoantibodies,
pubmed-meshheading:12297304-Autoantigens,
pubmed-meshheading:12297304-Brain,
pubmed-meshheading:12297304-Brain Diseases,
pubmed-meshheading:12297304-Electrophoresis, Gel, Two-Dimensional,
pubmed-meshheading:12297304-Humans,
pubmed-meshheading:12297304-Molecular Sequence Data,
pubmed-meshheading:12297304-Peptide Mapping,
pubmed-meshheading:12297304-Phosphopyruvate Hydratase,
pubmed-meshheading:12297304-Proteome,
pubmed-meshheading:12297304-Recombinant Proteins,
pubmed-meshheading:12297304-Spectrometry, Mass, Matrix-Assisted Laser...,
pubmed-meshheading:12297304-Thyroiditis, Autoimmune
|
|
pubmed:year |
2002
|
|
pubmed:articleTitle |
Proteomic analysis of human brain identifies alpha-enolase as a novel autoantigen in Hashimoto's encephalopathy.
|
|
pubmed:affiliation |
Department of Neurology, Neurological Institute, Graduate School of Medical Sciences, Kyushu University, Fukuoka 812-8582, Japan.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
