Linked Life Data

12296377

Source:http://linkedlifedata.com/resource/pubmed/id/12296377

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3-4
pubmed:dateCreated
2002-9-25
pubmed:abstractText
The human RNA methyltransferase like 1 gene (RNMTL1) is one of thirteen newly discovered genes within a 116 Kb segment of the chromosome 17p13.3 that suffers from a high frequent loss of heterozygosity in human hepatocellular carcinoma in China[1-5]. To understand the molecular mechanisms underlying transcription control of the RNMTL1 gene in human cancers, we decline using of the conventional approach where the cis-elements bound by the known transcription factors are primary targets, and carried out the systematic analyses to dissect the promoter structure and identify/characterize the key cis-elements that are responsible for its strong expression in cell. The molecular approaches applied included 1, the primer extension for mapping of the transcription starts; 2, the transient transfection/reporter assays on a large number of deletion and site-specific mutants of the promoter segment for defining the minimal promoter and the crucial elements within; and 3, the electrophoresis mobility shift assay with specific antibodies for reconfirming the nature of the transcription factors and their cognate cis-elements. We have shown that the interaction of an ATF/CREB element (-38 to -31) and its cognate transcription factors play a predominant role in the promoter activity of the RNMTL1 gene. The secondary DNA structures of the ATF/CREB element play a more vital role in the protein-DNA interaction. Finally, we reported a novel mechanism underlying the YY1 mediated transcription repression, namely, the ATF/CREB dependent transcription-repression by YY1 is executed in absence of its own sequence-specific binding.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
1001-0602
pubmed:author
pubmed:issnType
Print
pubmed:volume
12
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
177-97
pubmed:dateRevised
2010-2-4
pubmed:meshHeading
pubmed-meshheading:12296377-Activating Transcription Factors, pubmed-meshheading:12296377-Base Sequence, pubmed-meshheading:12296377-Binding Sites, pubmed-meshheading:12296377-Blood Proteins, pubmed-meshheading:12296377-Cell Line, pubmed-meshheading:12296377-Chromosomes, Human, Pair 17, pubmed-meshheading:12296377-Cyclic AMP Response Element-Binding Protein, pubmed-meshheading:12296377-Gene Deletion, pubmed-meshheading:12296377-Gene Expression, pubmed-meshheading:12296377-Genes, Reporter, pubmed-meshheading:12296377-Humans, pubmed-meshheading:12296377-Methyltransferases, pubmed-meshheading:12296377-Models, Genetic, pubmed-meshheading:12296377-Mutagenesis, Site-Directed, pubmed-meshheading:12296377-Promoter Regions, Genetic, pubmed-meshheading:12296377-RNA, pubmed-meshheading:12296377-Recombinant Proteins, pubmed-meshheading:12296377-Repressor Proteins, pubmed-meshheading:12296377-Transcription, Genetic, pubmed-meshheading:12296377-Transcription Factors, pubmed-meshheading:12296377-Tumor Cells, Cultured
pubmed:year
2002
pubmed:articleTitle
The ATF/CREB site is the key element for transcription of the human RNA methyltransferase like 1(RNMTL1) gene, a newly discovered 17p13.3 gene.
pubmed:affiliation
The State-key Laboratory for Oncogenes and Related Genes, Shanghai Cancer Institute, Xie-tu China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't