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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
7
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pubmed:dateCreated |
2002-9-23
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pubmed:abstractText |
Previously, we have shown in a model of hypersensitivity pneumonitis that Th1-biased C57BL/6 mice are susceptible and Th2-biased DBA/2 mice are resistant to disease. We also showed that this was explained in part by differential regulation of IL-12 by IL-4. For these reasons, we postulated that C57BL/6 and DBA/2 mice differentially express IL-4. In this study, we show that C57BL/6 immune cells express Th2 but not Th1 cytokines at lower levels than DBA/2 cells. We also found that C57BL/6 splenocytes exhibit decreased mRNA stability of Th2 cytokines, relative to DBA/2 splenocytes. Stability of IL-2 and IFN-gamma were similar in the two strains of mice. Differences in Th2 cytokine mRNA stability between C57BL/6 and DBA/2 cells were not due to sequence polymorphism at specific regions of the IL-4/IL-13 locus. Furthermore, expression of Th1- and Th2-specific transcription factors T-bet and GATA-3, as well as the nuclear factor of activated T cells transcription factor, NFATc, was not significantly different between the two mice. Our data suggest that decreased mRNA stability of Th2 cytokines in C57BL/6 splenocytes may underlie the differential susceptibility to hypersensitivity pneumonitis between C57BL/6 and DBA/2 mice. Moreover, our results indicate that regulation of mRNA stability may serve as an important mechanism underlying Th1/Th2 immune polarization.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/GATA3 Transcription Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Gata3 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Genetic Markers,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-4,
http://linkedlifedata.com/resource/pubmed/chemical/NFATC Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/T-Box Domain Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/T-box transcription factor TBX21,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0022-1767
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
169
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3700-9
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:12244163-Alveolitis, Extrinsic Allergic,
pubmed-meshheading:12244163-Animals,
pubmed-meshheading:12244163-Base Sequence,
pubmed-meshheading:12244163-CD4-Positive T-Lymphocytes,
pubmed-meshheading:12244163-Cells, Cultured,
pubmed-meshheading:12244163-DNA-Binding Proteins,
pubmed-meshheading:12244163-Disease Susceptibility,
pubmed-meshheading:12244163-Female,
pubmed-meshheading:12244163-GATA3 Transcription Factor,
pubmed-meshheading:12244163-Genetic Markers,
pubmed-meshheading:12244163-Immunophenotyping,
pubmed-meshheading:12244163-Interleukin-4,
pubmed-meshheading:12244163-Kinetics,
pubmed-meshheading:12244163-Lung,
pubmed-meshheading:12244163-Lymphocyte Count,
pubmed-meshheading:12244163-Mice,
pubmed-meshheading:12244163-Mice, Inbred C57BL,
pubmed-meshheading:12244163-Mice, Inbred DBA,
pubmed-meshheading:12244163-NFATC Transcription Factors,
pubmed-meshheading:12244163-Nuclear Proteins,
pubmed-meshheading:12244163-Polymorphism, Genetic,
pubmed-meshheading:12244163-RNA, Messenger,
pubmed-meshheading:12244163-RNA Stability,
pubmed-meshheading:12244163-Spleen,
pubmed-meshheading:12244163-T-Box Domain Proteins,
pubmed-meshheading:12244163-Th1 Cells,
pubmed-meshheading:12244163-Th2 Cells,
pubmed-meshheading:12244163-Trans-Activators,
pubmed-meshheading:12244163-Transcription Factors
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pubmed:year |
2002
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pubmed:articleTitle |
Altered IL-4 mRNA stability correlates with Th1 and Th2 bias and susceptibility to hypersensitivity pneumonitis in two inbred strains of mice.
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pubmed:affiliation |
Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, University of Iowa College of Medicine and Veterans Administration Medical Center, Iowa City, IA 52242, USA. noah-butler@uiowa.edu
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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