Linked Life Data

12242631

Source:http://linkedlifedata.com/resource/pubmed/id/12242631

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2002-9-20
pubmed:abstractText
A simple structured model is proposed for simulating batch cultivation data on growth, substrate utilization, and heterologous enzyme production of recombinant Saccharomyces cerevisiae YPB-G. The enzyme is a fusion protein displaying alpha-amylase and glucoamylase activities. Cell growth is modulated mainly by intracellular substrate and ethanol concentrations. Intracellular substrate concentration is evaluated by means of the extracellular substrate and biomass concentrations. Extracellular alpha-amylase and glucoamylase activities are taken to depend on biomass concentration. The nine parameters of the proposed model are determined using nonlinear estimation techniques, and the model is validated against experiments not used in parameter determination. The model developed simulates glucose consumption, cell mass, alpha-amylase and glucoamylase production in a batch system. Simulation and experimental results are found to be in good agreement.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
1367-5435
pubmed:author
pubmed:issnType
Print
pubmed:volume
29
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
111-6
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
2002
pubmed:articleTitle
A simple structured model for biomass and extracellular enzyme production with recombinant Saccharomyces cerevisiae YPB-G.
pubmed:affiliation
Department of Chemical Engineering, Bogaziçi University, 80815 Bebek, Istanbul, Turkey.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't