Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2002-9-19
pubmed:abstractText
Ependymomas, rare neoplasms of the central nervous system, occur predominantly in children. They are highly vascularized, and histological findings show many perivascular rosettes of tumoral cells radially organized around capillaries. Treatment of ependymomas relies on surgery combined with radio- or chemotherapy, but the efficiency of chemotherapy is limited, probably because of their multidrug resistance (MDR) phenotype. Progress in the therapy of these neoplasms is dramatically limited by the absence of cell line models. We established conditions for the long-term culture of human tumoral ependymocytes and their 3D coculture in Matrigel with endothelial cells. Histological, immunological, and ultrastructural studies showed that the morphological features (microvilli, cilia, and caveolae) of these cultured cells were similar to those of the tumor in vivo. The cells expressed potential oncological markers related to the immature state of tumoral cells (nestin and Notch-1), their tumorigenicity [caveolae and epidermal growth factor-receptor (EGF-R)], or the MDR phenotype [P-glycoprotein (P-gp)]. The expression of P-gp, EGF-R, and caveolin-1 by these tumoral ependymocytes could be useful in studies on new drugs. This coculture model might represent a new powerful tool to study new therapeutic delivery strategies in tumoral cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1019-6439
pubmed:author
pubmed:issnType
Print
pubmed:volume
21
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
775-85
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:12239616-Adolescent, pubmed-meshheading:12239616-Adult, pubmed-meshheading:12239616-Aged, pubmed-meshheading:12239616-Brain Neoplasms, pubmed-meshheading:12239616-Cell Culture Techniques, pubmed-meshheading:12239616-Cells, Cultured, pubmed-meshheading:12239616-Child, pubmed-meshheading:12239616-Child, Preschool, pubmed-meshheading:12239616-Coculture Techniques, pubmed-meshheading:12239616-Collagen, pubmed-meshheading:12239616-Drug Combinations, pubmed-meshheading:12239616-Endothelium, Vascular, pubmed-meshheading:12239616-Ependymoma, pubmed-meshheading:12239616-Female, pubmed-meshheading:12239616-Humans, pubmed-meshheading:12239616-Immunohistochemistry, pubmed-meshheading:12239616-Infant, pubmed-meshheading:12239616-Laminin, pubmed-meshheading:12239616-Male, pubmed-meshheading:12239616-Microscopy, Electron, pubmed-meshheading:12239616-Microscopy, Fluorescence, pubmed-meshheading:12239616-Middle Aged, pubmed-meshheading:12239616-P-Glycoprotein, pubmed-meshheading:12239616-Proteoglycans, pubmed-meshheading:12239616-Time Factors, pubmed-meshheading:12239616-Tumor Cells, Cultured, pubmed-meshheading:12239616-Umbilical Veins
pubmed:year
2002
pubmed:articleTitle
Establishment of human tumoral ependymal cell lines and coculture with tubular-like human endothelial cells.
pubmed:affiliation
Inserm U433, Faculté de Médecine R. Laennec, F-69372 Lyon, Cedex 08, France. brisson@laennec.univ-lyon1.fr
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't