Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6904
pubmed:dateCreated
2002-9-19
pubmed:abstractText
Messenger RNA regulation is a critical mode of controlling gene expression. Regulation of mRNA stability and translation is linked to controls of poly(A) tail length. Poly(A) lengthening can stabilize and translationally activate mRNAs, whereas poly(A) removal can trigger degradation and translational repression. Germline granules (for example, polar granules in flies, P granules in worms) are ribonucleoprotein particles implicated in translational control. Here we report that the Caenorhabditis elegans gene gld-2, a regulator of mitosis/meiosis decision and other germline events, encodes the catalytic moiety of a cytoplasmic poly(A) polymerase (PAP) that is associated with P granules in early embryos. Importantly, the GLD-2 protein sequence has diverged substantially from that of conventional eukaryotic PAPs, and lacks a recognizable RRM (RNA recognition motif)-like domain. GLD-2 has little PAP activity on its own, but is stimulated in vitro by GLD-3. GLD-3 is also a developmental regulator, and belongs to the Bicaudal-C family of RNA binding proteins. We suggest that GLD-2 is the prototype for a class of regulatory cytoplasmic PAPs that are recruited to specific mRNAs by a binding partner, thereby targeting those mRNAs for polyadenylation and increased expression.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0028-0836
pubmed:author
pubmed:issnType
Print
pubmed:day
19
pubmed:volume
419
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
312-6
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:12239571-Amino Acid Sequence, pubmed-meshheading:12239571-Animals, pubmed-meshheading:12239571-Caenorhabditis elegans, pubmed-meshheading:12239571-Caenorhabditis elegans Proteins, pubmed-meshheading:12239571-Catalytic Domain, pubmed-meshheading:12239571-Cloning, Molecular, pubmed-meshheading:12239571-Cytoplasm, pubmed-meshheading:12239571-Dimerization, pubmed-meshheading:12239571-Embryo, Nonmammalian, pubmed-meshheading:12239571-Evolution, Molecular, pubmed-meshheading:12239571-Germ-Line Mutation, pubmed-meshheading:12239571-Models, Biological, pubmed-meshheading:12239571-Models, Molecular, pubmed-meshheading:12239571-Molecular Sequence Data, pubmed-meshheading:12239571-Poly A, pubmed-meshheading:12239571-Polyadenylation, pubmed-meshheading:12239571-Polynucleotide Adenylyltransferase, pubmed-meshheading:12239571-Protein Binding, pubmed-meshheading:12239571-Protein Structure, Tertiary, pubmed-meshheading:12239571-RNA, Helminth, pubmed-meshheading:12239571-RNA, Messenger, pubmed-meshheading:12239571-RNA-Binding Proteins
pubmed:year
2002
pubmed:articleTitle
A regulatory cytoplasmic poly(A) polymerase in Caenorhabditis elegans.
pubmed:affiliation
Department of Biochemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't