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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
20
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pubmed:dateCreated |
2002-9-19
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pubmed:abstractText |
An important aspect of the pathophysiology of human immunodeficiency virus type 1 (HIV-1) infection is the ability of the virus to replicate in the host vigorously without a latent phase and to kill cells with a dynamic turnover of 1.8 x 10(9) cells/day and 10.3 x 10(9) virions/24 h. The transcription of HIV-1 RNA in acute infection occurs at two stages; the transcription of viral spliced mRNA occurs early, and the transcription of viral genomic RNA occurs later. The HIV-1 Tat protein is translated from the early spliced mRNA and is critical for HIV-1 genomic RNA expression. The cellular transcription factors are important for HIV-1 early spliced mRNA expression. In this study we show that virion nucleocapsid protein (NC) has a role in expression of HIV-1 early spliced mRNA. The HIV-1 NC migrates from the cytoplasm to the nucleus and accumulates in the nucleus at 18 h postinfection. Mutations on HIV-1 NC zinc fingers change the pattern of early viral spliced mRNA expression and result in a delayed expression of early viral mRNA in HIV-infected cells. This delayed HIV-1 early spliced mRNA expression occurs after proviral DNA has been integrated into the cellular genome, as shown by a quantitative integration assay. These results show that virion NC plays an important role in inducing HIV-1 early mRNA expression and contributes to the rapid viral replication that occurs during HIV-1 infection.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/12239321-10074138,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12239321-10087230,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12239321-10196217,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12239321-10341273,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12239321-10400764,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12239321-10704334,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12239321-10982342,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12239321-11329067,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12239321-1655281,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12239321-1678437,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12239321-1711215,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12239321-1731111,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12239321-2023953,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12239321-2448875,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12239321-2760980,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12239321-3016298,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12239321-3319186,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12239321-7529365,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12239321-7585960,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12239321-7729429,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12239321-7816094,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12239321-8156990,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12239321-8599114,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12239321-8648722,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12239321-8755517,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12239321-8849451,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12239321-9371822,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12239321-9372921,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12239321-9491887,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12239321-9601521,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12239321-9813197,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12239321-9813210,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12239321-9837967,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12239321-9878383
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Capsid Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Gene Products, gag,
http://linkedlifedata.com/resource/pubmed/chemical/NCP7 protein, Human...,
http://linkedlifedata.com/resource/pubmed/chemical/Nucleocapsid Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/gag Gene Products, Human...
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0022-538X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
76
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
10444-54
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:12239321-Capsid,
pubmed-meshheading:12239321-Capsid Proteins,
pubmed-meshheading:12239321-Cell Nucleus,
pubmed-meshheading:12239321-DNA, Viral,
pubmed-meshheading:12239321-Gene Expression,
pubmed-meshheading:12239321-Gene Products, gag,
pubmed-meshheading:12239321-HIV-1,
pubmed-meshheading:12239321-Humans,
pubmed-meshheading:12239321-Nucleocapsid Proteins,
pubmed-meshheading:12239321-RNA, Messenger,
pubmed-meshheading:12239321-RNA, Viral,
pubmed-meshheading:12239321-RNA Splicing,
pubmed-meshheading:12239321-Time Factors,
pubmed-meshheading:12239321-Tumor Cells, Cultured,
pubmed-meshheading:12239321-Viral Proteins,
pubmed-meshheading:12239321-Virus Integration,
pubmed-meshheading:12239321-gag Gene Products, Human Immunodeficiency Virus
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pubmed:year |
2002
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pubmed:articleTitle |
Human immunodeficiency virus type 1 nucleocapsid protein nuclear localization mediates early viral mRNA expression.
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pubmed:affiliation |
Division of Infectious Diseases, Dana 617, Beth Israel Deaconess Medical Center, Harvard Medical School, 330 Brookline Avenue, Boston, MA 02215, USA. lzhang@caregroup.harvard.edu
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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