Linked Life Data

12237843

Source:http://linkedlifedata.com/resource/pubmed/id/12237843

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2002-9-18
pubmed:abstractText
Recent advances in understanding oligodendrocyte development have revealed the importance of both extra- and intracellular molecules in regulating the induction, survival, and proliferation of early oligodendrocyte progenitors. The signaling molecule Sonic hedgehog (Shh) is critical for normal development of oligodendrocytes, although the precise influences of Shh on cells of the oligodendrocyte lineage are unclear. The present study shows that Shh increased the number of oligodendrocyte precursors in both pure cultures of oligodendrocyte precursors and mixed cultures from embryonic rat spinal cord. In pure precursor cultures Shh increased cell survival. In mixed cultures, Shh increased both the survival and proliferation of oligodendrocyte precursors in a concentration dependent manner. One intracellular consequence of exposure to Shh is the activation of transcription factors in oligodendrocyte lineage cells, which are critical for oligodendrocyte development, helix-loop-helix (HLH) transcription factors, Olig1 and 2. In many cases, HLH proteins such as Olig1 and Olig2 heterodimerize with other HLH proteins, such as members of the E subfamily, which are critical regulators of cell proliferation and differentiation. Immature (A2B5(+)) and more mature (O4(+)) rat oligodendrocyte precursors in dissociated cell culture expressed Olig1 as well as E proteins, HEB and E2A. Similarly, cells bearing the morphology of oligodendrocyte precursors expressed both Olig1 and HEB or E2A. We propose that E2A and/or HEB, possibly in combination with Olig1 and 2, are critical components of oligodendrogenesis and may regulate cell survival, proliferation, and fate decisions in the oligodendrocyte lineage.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0894-1491
pubmed:author
pubmed:copyrightInfo
Copyright 2002 Wiley-Liss, Inc.
pubmed:issnType
Print
pubmed:volume
40
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
55-64
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:12237843-Animals, pubmed-meshheading:12237843-Basic Helix-Loop-Helix Transcription Factors, pubmed-meshheading:12237843-Cell Communication, pubmed-meshheading:12237843-Cell Differentiation, pubmed-meshheading:12237843-Cell Division, pubmed-meshheading:12237843-Cell Lineage, pubmed-meshheading:12237843-Cell Survival, pubmed-meshheading:12237843-Cells, Cultured, pubmed-meshheading:12237843-Central Nervous System, pubmed-meshheading:12237843-DNA-Binding Proteins, pubmed-meshheading:12237843-Fetus, pubmed-meshheading:12237843-Gene Expression Regulation, Developmental, pubmed-meshheading:12237843-Hedgehog Proteins, pubmed-meshheading:12237843-Immunohistochemistry, pubmed-meshheading:12237843-Nerve Tissue Proteins, pubmed-meshheading:12237843-Oligodendroglia, pubmed-meshheading:12237843-Rats, pubmed-meshheading:12237843-Stem Cells, pubmed-meshheading:12237843-Trans-Activators, pubmed-meshheading:12237843-Transcription Factors
pubmed:year
2002
pubmed:articleTitle
Extracellular and intracellular regulation of oligodendrocyte development: roles of Sonic hedgehog and expression of E proteins.
pubmed:affiliation
Department of Neurosciences, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106-4975, USA. crs13@po.cwru.edu
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't