Linked Life Data

12234458

Source:http://linkedlifedata.com/resource/pubmed/id/12234458

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2002-9-17
pubmed:abstractText
cDNA microarray technology is becoming the technique of choice for studying gene expression and gene expression patterns. Although experimental protocols are available, only limited methodological information on microarray manufacture, hybridization, and signal interpretation has been published. The aim of this paper is to provide more insight into the practical aspects of microarray construction and hybridization. The influence of the size, composition, and concentration of the spotted DNA fragments on the final hybridization signal and the effect of hybridization volume, sample concentration, and sample depletion have been tested and are discussed.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0003-2697
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
308
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
5-17
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:12234458-Amines, pubmed-meshheading:12234458-Base Composition, pubmed-meshheading:12234458-Caco-2 Cells, pubmed-meshheading:12234458-Cloning, Molecular, pubmed-meshheading:12234458-DNA, Complementary, pubmed-meshheading:12234458-DNA Primers, pubmed-meshheading:12234458-Epithelial Cells, pubmed-meshheading:12234458-Genetic Vectors, pubmed-meshheading:12234458-Glass, pubmed-meshheading:12234458-Humans, pubmed-meshheading:12234458-Intestinal Mucosa, pubmed-meshheading:12234458-Luciferases, pubmed-meshheading:12234458-Oligonucleotide Array Sequence Analysis, pubmed-meshheading:12234458-Petunia, pubmed-meshheading:12234458-Poly A, pubmed-meshheading:12234458-Polymerase Chain Reaction, pubmed-meshheading:12234458-RNA, Messenger, pubmed-meshheading:12234458-Sensitivity and Specificity, pubmed-meshheading:12234458-Silanes, pubmed-meshheading:12234458-Substrate Specificity
pubmed:year
2002
pubmed:articleTitle
Factors influencing cDNA microarray hybridization on silylated glass slides.
pubmed:affiliation
RIKILT (State Institute for Quality Control of Agricultural Products), Gene Expression and Detection Centre, P.O. Box 230, 6700 AE Wageningen, The Netherlands. n.l.w.franssen@rikilt.wag-ur.nl
pubmed:publicationType
Journal Article